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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/188553

Preliminary study for simultaneous detection and quantification of androgenic anabolic steroids using ELISA and pattern recognition techniques

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A first step towards the multidetection, identification and quantification of anabolic androgenic steroids by enzyme-linked immunosorbent assay (ELISA) has been performed in this study. This proposal combines multiple competitive ELISA assays with different cross-reactivity profiles and multivariate data analysis techniques. Data have been analyzed by principal component analysis in conjunction with a novel K-nearest line classifier. This proposal allows simultaneous detection of up to four different steroids in the range of concentration from 0.1 to 316.2 nM with a total rate of 90.6% of correct detection, even in the presence of cross-reactivities. A methodology for concentration prediction is also presented with satisfactory results.

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CALVO, Daniel, TORT, Núria, SALVADOR VICO, Juan pablo, MARCO, M. pilar, CENTI, Fabiana, MARCO COLÁS, Santiago. Preliminary study for simultaneous detection and quantification of androgenic anabolic steroids using ELISA and pattern recognition techniques. _Analyst_. 2011. Vol. 136, núm. 19, pàgs. 4045-4052. [consulta: 25 de febrer de 2026]. ISSN: 0003-2654. [Disponible a: https://hdl.handle.net/2445/188553]

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