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cc-by (c) de Salazar, Lydia et al., 2020
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/172471

Oxidative stress in endurance cycling is reduced dose-dependently after one month of re-esterified DHA supplementation

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Docosahexaenoic acid (DHA) supplementation can reduce exercise-induced oxidative stress generated during long aerobic exercise, with the minimum dose yet to be elucidated for physically active subjects. In this study, we performed a dose finding with re-esterified DHA in triglyceride form in a randomized double-blind parallel trial at different doses (350, 1050, 1750, and 2450 mg a day) for 4 weeks in males engaged in regular cycling (n = 100, 7.6 ± 3.7 h/week). The endogenous antioxidant capacity of DHA was quantified as a reduction in the levels of the oxidative stress marker 8-hydroxy-2'-deoxyguanosine (8-OHdG) recollected in 24-h urine samples after 90 min of constant load cycling before and after intervention. To ascertain incorporation of DHA, erythrocyte polyunsaturated fatty acid (PUFA) composition was compared along groups. We found a dose-dependent antioxidant capacity of DHA from 1050 mg with a trend to neutralization for the highest dose of 2450 mg (placebo: n = 13, F = 0.041; 350 mg: n = 10, F = 0.268; 1050 mg: n = 11, F = 7.112; 1750 mg: n = 12, F = 9.681; 2450 mg: n = 10, F = 15.230). In the erythrocyte membrane, the re-esterified DHA increased DHA and omega-3 percentage and decreased omega 6 and the omega-6 to omega-3 ratio, while Eicosapentaenoic acid (EPA) and PUFA remained unchanged. Supplementation of re-esterified DHA exerts a dose-dependent endogenous antioxidant property against moderate-intensity long-duration aerobic exercise in physically active subjects when provided at least 1050 mg a day for 4 weeks.

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DE SALAZAR, Lydia, TORREGROSA-GARCIA, Antonio, LUQUE-RUBIA, Antonio j., AVILA-GANDIA, Vicente, DOMINGO I PEDROL, Joan carles, LÓPEZ ROMÁN, Fco. javier. Oxidative stress in endurance cycling is reduced dose-dependently after one month of re-esterified DHA supplementation. _Antioxidants_. 2020. Vol. 9, núm. 11, pàgs. 1145. [consulta: 25 de febrer de 2026]. ISSN: 2076-3921. [Disponible a: https://hdl.handle.net/2445/172471]

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