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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/230156
Universal cold RNA phase transitions
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RNA’s diversity of structures and functions impacts all life forms since primordia. We use calorimetric force spectroscopy to investigate RNA folding landscapes in previously unexplored low-temperature conditions. We find that Watson–Crick RNA hairpins, the most basic secondary structure elements, undergo a glass-like transition below TG ∼ 20 ◦C where the heat capacity abruptly changes and the RNA folds into a diversity of misfolded structures. We hypothesize that an altered RNA biochemistry, determined by sequence-independent ribose–water interactions, outweighs sequence-dependent base pairing. The ubiquitous ribose–water interactions lead to universal
RNA phase transitions below TG, such as maximum stability at TS ∼ 5 ◦C where water density is maximum, and cold denaturation at TC ∼ −50 ◦C. RNA cold biochemistry may have a profound impact on RNA function and evolution.
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RISSONE, Paolo, et al. Universal cold RNA phase transitions. Proceedings of the National Academy of Sciences of the United States of America - PNAS. 2024. Vol. 121, num. 34. ISSN 0027-8424. [consulted: 11 of July of 2026]. Available at: https://hdl.handle.net/2445/230156