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Fitxers

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2020-06-18

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cc-by (c) Arato, Ivo et al., 2020
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/176569

'In vitro' Effect of Different Follicle¿Stimulating Hormone Preparations on Sertoli Cells: Toward a Personalized Treatment for Male Infertility

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Recurs relacionat

https://doi.org/10.3389/fendo.2020.00401

Resum

Follicle-stimulating hormone (FSH), a major regulator of spermatogenesis, has a crucial function in the development and function of the testis and it is extensively given as a fertility treatment to stimulate spermatogenesis. We analyzed the effects of different FSH preparations (α-follitropin, β-follitropin, and urofollitropin) in combination with testosterone on porcine pre-pubertal Sertoli cells. To study the effect of the different FSH treatments in the Sertoli cell function we performed Real Time PCR analysis of AMH, inhibin B, and FSH-r, an ELISA assay for AMH and inhibin B, and a high-throughput comparative proteomic analysis. We verified that all three preparations induced a reduction of AMH in terms of mRNA and secreted proteins, and an increase of inhibin B in terms of mRNA in all the FSH formulations, while solely α-follitropin produced an increase of secreted inhibin B in the culture medium. Comparative proteomic analysis of the three FSH preparations identified 46 proteins, 11 up-regulated and 2 down-regulated. Surprisingly, the combination of testosterone with β-follitropin specifically induced an up-regulation of eight specific secreted proteins. Our study, showing that the three different FSH preparations induce different effects, could offer the opportunity to shed light inside new applications to a personalized reproductive medicine.

Matèries

Cèl·lules de Sertoli, Espermatogènesi

Matèries (anglès)

Sertoli cells, Spermatogenesis

Citació

Col·leccions

Articles publicats en revistes (Biomedicina)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)

Citació

ARATO, Ivo, et al. 'In vitro' Effect of Different Follicle¿Stimulating Hormone Preparations on Sertoli Cells: Toward a Personalized Treatment for Male Infertility. Frontiers In Endocrinology. 2020. Vol. 11, num. 401. ISSN 1664-2392. [consulted: 4 of August of 2026]. Available at: https://hdl.handle.net/2445/176569

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