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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/190755
The relevance of EGFR, ErbB receptors and neuregulins in human adipocytes and adipose tissue in obesity
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Objective: To investigate the potential role of EGFR, ErbBs receptors and neuregulins in human adipose tissue physiology in obesity. Methods: Gene expression analysis in human subcutaneous (SAT) and visceral (VAT) adipose tissue in three independent cohorts [two cross-sectional (N = 150, N = 87) and one longitudinal (n = 25)], and in vitro gene knockdown and overexpression experiments were performed. Results: While both SAT and VAT ERBB2 and ERBB4 mRNA increased in obesity, SAT EGFR mRNA was negatively correlated with insulin resistance, but did not change in obesity. Of note, both SAT and VAT EGFR mRNA were significantly associated with adipogenesis and increased during human adipocyte differentiation. In vitro experiments revealed that EGFR, but not ERBB2 and ERBB4, gene knockdown in preadipocytes and in fully differentiated human adipocytes resulted in decreased expression of adipogenic-related genes. ERBB2 gene knockdown also reduced gene expression of fatty acid synthase in fully differentiated adipocytes. In addition, neuregulin 2 (NRG2) mRNA was associated with expression of adipogenic genes in human adipose tissue and adipocytes, and its overexpression increased expression of EGFR and relevant adipogenic genes. Conclusions: This study demonstrates the association between adipose tissue ERBB2 and obesity, confirms the relevance of EGFR on human adipogenesis, and suggests a possible adipogenic role of NRG2.
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LATORRE, Jèssica, MARTÍNEZ, Cristina, ORTEGA, Francisco, OLIVERAS-CAÑELLAS, Núria, DÍAZ-SÁEZ, Francisco, ARAGONÉS, Julián, CAMPS CAMPRUBÍ, Marta, GUMÀ I GARCIA, Anna maria, RICART, Wifredo, FERNÁNDEZ-REAL LEMOS, José manuel, MORENO-NAVARRETE, José maría. The relevance of EGFR, ErbB receptors and neuregulins in human adipocytes and adipose tissue in obesity. _Biomedicine & Pharmacotherapy_. 2022. Vol. 156, núm. 113972. [consulta: 23 de gener de 2026]. ISSN: 0753-3322. [Disponible a: https://hdl.handle.net/2445/190755]