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Accepted version

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2016-02-08

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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/113749

Metabolic profiling for the identification of Huntington biomarkers by on-line solid-phase extraction capillary electrophoresis mass spectrometry combined with advanced data analysis tools

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https://doi.org/10.1002/elps.201500378

Abstract

In this work, an untargeted metabolomic approach based on sensitive analysis by on-line solid-phase extraction capillary electrophoresis mass spectrometry (SPE-CE-MS) in combination with multivariate data analysis is proposed as an efficient method for the identification of biomarkers of Huntington's disease (HD) progression in plasma. For this purpose, plasma samples from wild type (wt) and HD (R6/1) mice of different ages (8, 12 and 30 weeks), were analysed by C18-SPE-CE-MS in order to obtain the characteristic electrophoretic profiles of low molecular mass compounds. Then, multivariate curve resolution alternating least squares (MCR-ALS) was applied to the multiple full scan MS data sets. This strategy permitted the resolution of a large number of metabolites being characterised by their electrophoretic peaks and their corresponding mass spectra. A total number of 29 compounds were relevant to discriminate between wt and HD plasma samples, as well as to follow-up the HD progression. The intracellular signalling was found to be the most affected metabolic pathway in HD mice after 12 weeks of birth, when mice already showed motor coordination deficiencies and cognitive decline. This fact agreed with the atrophy and dysfunction of specific neurons, loss of several types of receptors and changed expression of neurotransmitters.

Subject

Marcadors bioquímics, Espectrometria de masses, Gestors de dades, Corea de Huntington

Subject (English)

Biochemical markers, Mass spectrometry, Data warehousing, Huntington's chorea

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Articles publicats en revistes (Enginyeria Química i Química Analítica)

Citation

PONT VILLANUEVA, Laura, et al. Metabolic profiling for the identification of Huntington biomarkers by on-line solid-phase extraction capillary electrophoresis mass spectrometry combined with advanced data analysis tools. Electrophoresis. 2016. Vol. 37, num. 5-6, pags. 795-808. ISSN 0173-0835. [consulted: 9 of August of 2026]. Available at: https://hdl.handle.net/2445/113749

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