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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/199282
SPR biosensor for BSA protein detection
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Abstract
For the last decades Surface Plasmon Resonance (SPR) biosensors have been revolutionizing the
world of optical biosensing techniques making great impact in the fields of drug discovery,
environmental monitoring, food safety, and medical diagnostics. In this project a grating coupled
SPR biosensor is analyzed by detecting a model protein, Bovine Serum Albumin (BSA).
The main goal is to assess the sensing capability, for that, three experimental approaches are
followed. First, a characterization of the biosensor’s surface is performed, and the surface is
visualized through images with a Surface electron microscope (SEM), which uses electrons to
generate images at the nanoscale (10-9 m). The second block, consist of performing several
experiments to detect the model protein, this will also allow to gain knowledge on the use of SPR
for protein detection. The last block is to repeat the previous experiments but this time altering the
surface of the biosensor to enhance a more specific binding of the protein to the surface. Changing
the surface properties is widely used in biosensing and it is called functionalizing. To ensure that
this functionalization is done correctly Fluorescence Microscopy, which helps to distinguish different
structures on a sample, is used.
Description
Treballs Finals de Grau d'Enginyeria Biomèdica. Facultat de Medicina i Ciències de la Salut. Universitat de Barcelona. Curs: 2022-2023. Tutor/Director: Moreno Sereno, Mauricio, Caballero Hernández, Ana Belén
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SOLÀ BOSCH, Carla. SPR biosensor for BSA protein detection. [consulted: 19 of August of 2026]. Available at: https://hdl.handle.net/2445/199282