The Effect of Small Cosolutes that Mimic Molecular Crowding Conditions on the Stability of Triplexes Involving Duplex DNA

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dc.contributor.authorAviñó Andrés, Anna
dc.contributor.authorMazzini, Stefania
dc.contributor.authorGargallo Gómez, Raimundo
dc.contributor.authorEritja i Casadellà, Ramon
dc.date.accessioned2020-03-03T11:14:22Z
dc.date.available2020-03-03T11:14:22Z
dc.date.issued2016-02-05
dc.date.updated2020-03-03T11:14:22Z
dc.description.abstractTriplex stability is studied in crowding conditions using small cosolutes (ethanol, acetonitrile and dimethylsulfoxide) by ultraviolet (UV), circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopies. The results indicate that the triplex is formed preferentially when the triplex forming oligonucleotide (TFO) is RNA. In addition, DNA triplexes (D:D·D) are clearly less stable in cosolute solutions while the stability of the RNA triplexes (R:D·D) is only slightly decreased. The kinetic of triplex formation with RNA-TFO is slower than with DNA-TFO and the thermal stability of the triplex is increased with the salt concentration in EtOH-water solutions. Accordingly, RNA could be considered a potential molecule to form a stable triplex for regulatory purposes in molecular crowding conditions.
dc.format.extent13 p.
dc.format.mimetypeapplication/pdf
dc.identifier.idgrec656922
dc.identifier.issn1661-6596
dc.identifier.pmid26861295
dc.identifier.urihttps://hdl.handle.net/2445/151798
dc.language.isoeng
dc.publisherMDPI
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.3390/ijms17020211
dc.relation.ispartofInternational Journal of Molecular Sciences, 2016, vol. 17, num. 2, p. 211
dc.relation.urihttps://doi.org/10.3390/ijms17020211
dc.rightscc-by (c) Aviñó, Anna et al., 2016
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es
dc.sourceArticles publicats en revistes (Enginyeria Química i Química Analítica)
dc.subject.classificationOligonucleòtids
dc.subject.classificationADN
dc.subject.classificationRNA
dc.subject.otherOligonucleotides
dc.subject.otherDNA
dc.subject.otherRNA
dc.titleThe Effect of Small Cosolutes that Mimic Molecular Crowding Conditions on the Stability of Triplexes Involving Duplex DNA
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion

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