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2022-11-01

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cc-by-nc-nd (c) Elsevier B.V., 2022
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/225668

Malaria quantitative POC testing using magnetic particles, a paper microfluidic device and a hand-held fluorescence reader

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https://doi.org/10.1016/j.bios.2022.114513

Resum

A point-of-care (POC) device is reported for highly sensitive and selective detection of Plasmodium falciparum lactate dehydrogenase (Pf-LDH), a biomarker of malaria infection, based on a single-step magneto-immunoassay, a single-use microfluidic paper device and a customized hand-held fluorescence reader. The single-step magneto-immunoassay consists in a single 5-min incubation of immuno-modified magnetic particles (c-MAb-MPs), biotinylated detection antibody (bd-MAb), and an enzymatic signal amplifier (Poly-HRP). After on-chip MP concentration and washing, signal generation is achieved by adding a fluorescent enzymatic substrate (QuantaRed). Fluorescence signal is measured using a low-cost customized, portable, and sensible fluorescent detector. The POC affords quantitative Pf-LDH detection in <20 min, with a detection limit of 0.92 ng mL−1 (equivalent to 4.6 parasites μL−1). Furthermore, Pf-LDH quantitation in clinical samples correlates with that provided by the reference ELISA, is more sensitive than a commercial rapid diagnostic test (RDT) and entails little user intervention. These results show that fluorescent paper-based microfluidic devices can be exploited to simplify magneto-immunoassay handling, taking this type of test closer to the requirements of POC testing.

Matèries

Plasmodium falciparum, Malària, Diagnòstic

Matèries (anglès)

Plasmodium falciparum, Malaria, Diagnosis

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Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)
Articles publicats en revistes (Institut de Nanociència i Nanotecnologia (IN2UB))

Citació

ARIAS ALPÍZAR, Kevin, et al. Malaria quantitative POC testing using magnetic particles, a paper microfluidic device and a hand-held fluorescence reader. Biosensors and Bioelectronics. 2022. Vol. 215, num. 114513. ISSN 0956-5663. [consulted: 2 of June of 2026]. Available at: https://hdl.handle.net/2445/225668

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