Amb motiu del tancament d'estiu, la validació de documents es reprendrà a partir del 28 d'agost de 2026. Disculpeu les molèsties.
Con motivo del cierre de verano, la validación de documentos se reanudará a partir del 28 de agosto de 2026. Disculpad las molestias
Due to the summer closure, document validation will resume starting August 28, 2026. We apologize for any inconvenience.

Files

672640.pdf (1.4 MB)

Document type

Article

Version

Published version

Publication date

2017-06-12

Publication license

cc-by-nc (c) Sans-Serramitjana, Eulalia et al., 2017
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/116850

Determination of the spatiotemporal dependence of Pseudomonas aeruginosa biofilm viability after treatment with NLC-colistin

Journal Title

Authors

Director/Tutor

Journal ISSN

Volume Title

Related resource

https://doi.org/10.2147/IJN.S138763

Abstract

The emergence of colistin-resistant Pseudomonas aeruginosa in cystic fibrosis (CF) patients, particularly after long-term inhalation treatments, has been recently reported. Nanoencapsulation may enable preparations to overcome the limitations of conventional pharmaceutical forms. We have determined the time-dependent viability of P. aeruginosa biofilms treated with both free and nanoencapsulated colistin. We also examined the relationship between the optimal anti-biofilm activity of nanostructured lipid carrier (NLC)-colistin and the structural organization of the biofilm itself. The results showed the more rapid killing of P. aeruginosa bacterial biofilms by NLC-colistin than by free colistin. However, the two formulations did not differ in terms of the final percentages of living and dead cells, which were higher in the inner than in the outer layers of the treated biofilms. The effective anti-biofilm activity of NLC-colistin and its faster killing effect recommend further studies of its use over free colistin in the treatment of P. aeruginosa infections in CF patients.

Subject

Pseudomonas, Antibiòtics, Biofilms, Fibrosi quística, Microscòpia confocal, Nanomedicina

Subject (English)

Pseudomonas, Antibiotics, Biofilms, Cystic fibrosis, Confocal microscopy, Nanomedicine

Citation

Collections

Articles publicats en revistes (Patologia i Terapèutica Experimental)

Citation

SANS SERRAMITJANA, Eulàlia, et al. Determination of the spatiotemporal dependence of Pseudomonas aeruginosa biofilm viability after treatment with NLC-colistin. International Journal of Nanomedicine. 2017. Vol. 12, num. 4409-4413. ISSN 1176-9114. [consulted: 13 of August of 2026]. Available at: https://hdl.handle.net/2445/116850

Export metadata

JSON - METS

Share record