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2017-01-11

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cc-by (c) Álvarez-González, Begoña et al., 2017
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/228025

Two-layer elastographic 3-D traction force microscopy

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Recurs relacionat

https://doi.org/10.1038/srep39315

Resum

Cellular traction force microscopy (TFM) requires knowledge of the mechanical properties of the substratum where the cells adhere to calculate cell-generated forces from measurements of substratum deformation. Polymer-based hydrogels are broadly used for TFM due to their linearly elastic behavior in the range of measured deformations. However, the calculated stresses, particularly their spatial patterns, can be highly sensitive to the substratum’s Poisson’s ratio. We present two-layer elastographic TFM (2LETFM), a method that allows for simultaneously measuring the Poisson’s ratio of the substratum while also determining the cell-generated forces. The new method exploits the analytical solution of the elastostatic equation and deformation measurements from two layers of the substratum. We perform an in silico analysis of 2LETFM concluding that this technique is robust with respect to TFM experimental parameters, and remains accurate even for noisy measurement data. We also provide experimental proof of principle of 2LETFM by simultaneously measuring the stresses exerted by migrating Physarum amoeboae on the surface of polyacrylamide substrata, and the Poisson’s ratio of the substrata. The 2LETFM method could be generalized to concurrently determine the mechanical properties and cell-generated forces in more physiologically relevant extracellular environments, opening new possibilities to study cell-matrix interactions.

Matèries

Elasticitat, Microscòpia, Cèl·lules

Matèries (anglès)

Elasticity, Microscopy, Cells

Citació

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Articles publicats en revistes (Genètica, Microbiologia i Estadística)

Citació

ÁLVAREZ-GONZÁLEZ, Begoña, et al. Two-layer elastographic 3-D traction force microscopy. Scientific Reports. 2017. Vol. 7, num. 39315, pags. 1-15. ISSN 2045-2322. [consulted: 17 of May of 2026]. Available at: https://hdl.handle.net/2445/228025

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