Document type
ArticleVersion
Accepted versionPublication date
All rights reserved
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/192916
The combined use of gold nanoparticles and infrared radiation enables cytosolic protein delivery
Journal Title
Director/Tutor
Additional contribution
Journal ISSN
Volume Title
Abstract
Cytosolic protein delivery remains elusive. The inability of most proteins to cross the cellular membrane is a huge hurdle. Here we explore the unique photothermal properties of gold nanorods (AuNRs) to trigger cytosolic delivery of proteins. Both partners, protein and AuNRs, are modified with a protease-resistant cell-penetrating peptide with nuclear targeting properties to induce internalization. Once internalised, spatiotemporal control of protein release is achieved by near-infrared laser irradiation in the safe second biological window. Importantly, catalytic amounts of AuNRs are sufficient to trigger cytosolic protein delivery. To the best of our knowledge, this is the first time that AuNRs with their maximum of absorption in the second biological window are used to deliver proteins into the intracellular space. This strategy represents a powerful tool for the cytosolic delivery of virtually any class of protein.
Related resource
Subject
Subject (English)
Citation
Citation
GARCIA, Josep, et al. The combined use of gold nanoparticles and infrared radiation enables cytosolic protein delivery. Chemistry-A European Journal. 2020. Vol. 27, num. 4670-4675. ISSN 0947-6539. [consulted: 18 of October of 2026]. Available at: https://hdl.handle.net/2445/192916