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Cells as active particles in asymmetric potentials: Motility under external gradients

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Cell migration is a crucial event during development and in disease. Mechanical constraints and chemical gradients can contribute to the establishment of cell direction, but their respective roles remain poorly understood. Using a microfabricated topographical ratchet, we show that the nucleus dictates the direction of cell movement through mechanical guidance by its environment. We demonstrate that this direction can be tuned by combining the topographical ratchet with a biochemical gradient of fibronectin adhesion. We report competition and cooperation between the two external cues. We also quantitatively compare the measurements associated with the trajectory of a model that treats cells as fluctuating particles trapped in a periodic asymmetric potential. We show that the cell nucleus contributes to the strength of the trap, whereas cell protrusions guided by the adhesive gradients add a constant tunable bias to the direction of cell motion.

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COMELLES PUJADAS, Jordi, CABALLERO VILA, David, VOITURIEZ, Raphaël, HORTIGÜELA, Verónica, WOLLRAB, Viktoria, GODEAU, Amélie luise, SAMITIER I MARTÍ, Josep, MARTÍNEZ FRAIZ, Elena, RIVELINE, Daniel. Cells as active particles in asymmetric potentials: Motility under external gradients. _Biophysical Journal_. 2014. Vol. 107, núm. 7, pàgs. 1513-1522. [consulta: 26 de febrer de 2026]. ISSN: 0006-3495. [Disponible a: https://hdl.handle.net/2445/68349]

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