Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/192216
Active DNA demethylation of developmental cis-regulatory regions predates vertebrate origins
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
DNA methylation [5-methylcytosine (5mC)] is a repressive gene-regulatory mark required for vertebrate embryogenesis. Genomic 5mC is tightly regulated through the action of DNA methyltransferases, which deposit 5mC, and ten-eleven translocation (TET) enzymes, which participate in its active removal through the formation of 5-hydroxymethylcytosine (5hmC). TET enzymes are essential for mammalian gastrulation and activation of vertebrate developmental enhancers; however, to date, a clear picture of 5hmC function, abundance, and genomic distribution in nonvertebrate lineages is lacking. By using base-resolution 5mC and 5hmC quantification during sea urchin and lancelet embryogenesis, we shed light on the roles of nonvertebrate 5hmC and TET enzymes. We find that these invertebrate deuterostomes use TET enzymes for targeted demethylation of regulatory regions associated with developmental genes and show that the complement of identified 5hmC-regulated genes is conserved to vertebrates. This work demonstrates that active 5mC removal from regulatory regions is a common feature of deuterostome embryogenesis suggestive of an unexpected deep conservation of a major gene-regulatory module.
Matèries
Matèries (anglès)
Citació
Citació
SKVORTSOVA, Ksenia, BERTRAND, Stephanie, VORONOV, Danila, DUCKETT, Paul e., ROSS, Samuel e., MAGRI, Marta silvia, MAESO, Ignacio, WEATHERITT, Robert j., GÓMEZ SKARMETA, Jose luis, ARNONE, Maria ina, ESCRIVA, Hector, BOGDANOVIC, Ozren. Active DNA demethylation of developmental cis-regulatory regions predates vertebrate origins. _Science Advances_. 2022. Vol. 8, núm. 48. [consulta: 20 de gener de 2026]. ISSN: 2375-2548. [Disponible a: https://hdl.handle.net/2445/192216]