Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/220281
Decompensated MASH-cirrhosis model by acute and toxic effects of phenobarbital.
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
Metabolic dysfunction-associated Steatohepatitis (MASH), is a prominent cause for liver cirrhosis. MASH-cirrhosis is responsible for liver complications and there is no specific treatment. To develop new therapeutic approaches, animal models are needed. The aim of this study was to develop a fast animal model of MASH-cirrhosis in rats reflecting the human disease. Carbon tetrachloride (CCl4) injections in combination with a high-fat Western diet (WD) were used to induce MASH-cirrhosis. To accelerate liver injury, animals received phenobarbital (PB) in their drinking water using two different regimens. Rats developed advanced MASH-cirrhosis characterized by portal hypertension, blood biochemistry, hepatic ballooning, steatosis, inflammation and fibrosis. Importantly, rats receiving low-dose PB for the long term (LT) showed ascites after 6 weeks, whereas rats with high-dose short-term (ST) PB developed ascites after 8 weeks. ST- and LT-treated rats showed increased portal pressure (PP) and decreased mean arterial pressure (MAP). Of note, hepatocyte ballooning was only observed in the LT group. The LT administration of low-dose PB with CCl4 intoxication and WD represents a fast and reproducible rat model mimicking decompensated MASH-cirrhosis in humans. Thus, CCl4 + WD with LT low-dose phenobarbital treatment might be the preferred rat animal model for drug development in MASH-cirrhosis.
Matèries (anglès)
Citació
Citació
GRÜNEWALD, Inga, KRAUS, Nico, USCHNER, Frank erhard, MOESLEIN, Magnus, SCHIERWAGEN, Robert, GU, Wenyi, BROL, Maximilian joseph, FÜRST, Eike, LOTERSZTAJN, Sophie, RAUTOU, Pierre-emmanuel, DURAN GÜELL, Marta, FLORES COSTA, Roger, CLÀRIA I ENRICH, Joan, TREBICKA, Jonel, KLEIN, Sabine. Decompensated MASH-cirrhosis model by acute and toxic effects of phenobarbital.. _Cells_. 2024. Vol. 13, núm. 20. [consulta: 28 de gener de 2026]. ISSN: 2073-4409. [Disponible a: https://hdl.handle.net/2445/220281]