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2014-06-09

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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/58572

Method development for the simultaneous determination of methylmercury and inorganic mercury in seafood

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http://dx.doi.org/10.1016/j.foodcont.2014.05.054

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This paper reports the method development for the simultaneous determination of methylmercury MeHgþ) and inorganic mercury (iHg) species in seafood samples. The study focused on the extraction and quantification of MeHgþ (the most toxic species) by liquid chromatography coupled to on-line UV irradiation and cold vapour atomic fluorescence spectroscopy (LC-UV-CV-AFS), using HCl 4 mol/L as the extractant agent. Accuracy of the method has been verified by analysing three certified reference materials and different spiked samples. The values found for total Hg and MeHgþ for the CRMs did not differ significantly from certified values at a 95% confidence level, and recoveries between 85% and 97% for MeHgþ, based on spikes, were achieved. The detection limits (LODs) obtained were 0.001 mg Hg/kg for total mercury, 0.0003 mg Hg/kg for MeHgþ and 0.0004 mg Hg/kg for iHg. The quantification limits (LOQs) established were 0.003 mg Hg/kg for total mercury, 0.0010 mg Hg/kg for MeHgþ and 0.0012 mg Hg/kg for iHg. Precision for each mercury species was established, being 12% in terms of RSD in all cases. Finally, the developed method was applied to 24 seafood samples from different origins and total mercury contents. The concentrations for Total Hg, MeHg and iHg ranged from 0.07 to 2.33, 0.003-2.23 and 0.006-0.085 mg Hg/kg, respectively. The established analytical method allows to obtain results for mercury speciation in less than 1 one hour including both, sample pretreatment and measuring step.

Matèries

Mercuri, Marisc, Tests de toxicitat, Química analítica

Matèries (anglès)

Mercury, Seafood, Toxicity testing, Analytical chemistry

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Articles publicats en revistes (Enginyeria Química i Química Analítica)

Citació

ZMOZINSKI, Ariane v., CARNEADO MORENO, Sergio, IBÁÑEZ PALOMINO, Carmen, SAHUQUILLO ESTRUGO, Àngels, LÓPEZ SÁNCHEZ, José fermín, DA SILVA, Márcia m.. Method development for the simultaneous determination of methylmercury and inorganic mercury in seafood. _Food Control_. 2014. Vol. 46, núm. 351-359. [consulta: 13 de gener de 2026]. ISSN: 0956-7135. [Disponible a: https://hdl.handle.net/2445/58572]

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