Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió acceptadaData de publicació
Tots els drets reservats
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/192215
A conserved Shh cis-regulatory module highlights a common developmental origin of unpaired and paired fins
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
Despite their evolutionary, developmental, and functional importance the origin of vertebrate paired appendages remains uncertain. In mice, a single enhancer termed ZRS is solely responsible for Shh expression in limbs. Here, zebrafish and mouse transgenic assays trace the functional equivalence of ZRS across the gnathostome phylogeny. CRISPR/Cas9-mediated deletion of the medaka-ZRS and enhancer assays reveal the existence of ZRS shadow enhancers in both teleost and human genomes. Deletion of both ZRS and shadow ZRS abolish shh expression and completely truncate pectoral fin formation. Strikingly, deletion of ZRS results in an almost complete ablation of the dorsal fin. This finding indicates that a ZRS-Shh regulatory module is shared by paired and median fins, and that paired fins likely emerged by the co‐option of developmental programs established in the median fins of stem gnathostomes. Shh function was later reinforced in pectoral fin development with the recruitment of shadow enhancers, conferring additional robustness.
Matèries
Matèries (anglès)
Citació
Citació
LETELIER, Joaquín, DE LA CALLE-MUSTIENES, Elisa, PIERETTI, Joyce, NARANJO, Silvia, MAESO, Ignacio, NAKAMURA, Tetsuya, PASCUAL-ANAYA, Juan, SHUBIN, Neil h, IGOR, Schneider, MARTINEZ-MORALES, Juan ramón, GÓMEZ-SKARMETA, José luis. A conserved Shh cis-regulatory module highlights a common developmental origin of unpaired and paired fins. _Nature Genetics_. 2018. Vol. 50, núm. 4, pàgs. 504-509. [consulta: 21 de gener de 2026]. ISSN: 1061-4036. [Disponible a: https://hdl.handle.net/2445/192215]