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Published version

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2013-12-23

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cc-by (c) Kassan, A. et al., 2013
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/54946

Acyl-CoA synthetase 3 promotes lipid droplet biogenesis in ER microdomains.

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http://dx.doi.org/10.1083/jcb.201305142

Abstract

Control of lipid droplet (LD) nucleation and copy number are critical, yet poorly understood, processes. We use model peptides that shift from the endoplasmic reticulum (ER) to LDs in response to fatty acids to characterize the initial steps of LD formation occurring in lipid-starved cells. Initially, arriving lipids are rapidly packed in LDs that are resistant to starvation (pre-LDs). Pre-LDs are restricted ER microdomains with a stable core of neutral lipids. Subsequently, a first round of"emerging" LDs is nucleated, providing additional lipid storage capacity. Finally, in proportion to lipid concentration, new rounds of LDs progressively assemble. Confocal microscopy and electron tomography suggest that emerging LDs are nucleated in a limited number of ER microdomains after a synchronized stepwise process of protein gathering, lipid packaging, and recognition by Plin3 and Plin2. A comparative analysis demonstrates that the acyl-CoA synthetase 3 is recruited early to the assembly sites, where it is required for efficient LD nucleation and lipid storage.

Subject

Coenzims, Metabolisme dels lípids, Transport biològic

Subject (English)

Coenzymes, Lipid metabolism, Biological transport

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Articles publicats en revistes (Biomedicina)

Citation

KASSAN, Adam, et al. Acyl-CoA synthetase 3 promotes lipid droplet biogenesis in ER microdomains. Journal of Cell Biology. 2013. Vol. 203, num. 6, pags. 985-1001. ISSN 0021-9525. [consulted: 12 of June of 2026]. Available at: https://hdl.handle.net/2445/54946

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