Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/54946
Acyl-CoA synthetase 3 promotes lipid droplet biogenesis in ER microdomains.
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
Control of lipid droplet (LD) nucleation and copy number are critical, yet poorly understood, processes. We use model peptides that shift from the endoplasmic reticulum (ER) to LDs in response to fatty acids to characterize the initial steps of LD formation occurring in lipid-starved cells. Initially, arriving lipids are rapidly packed in LDs that are resistant to starvation (pre-LDs). Pre-LDs are restricted ER microdomains with a stable core of neutral lipids. Subsequently, a first round of"emerging" LDs is nucleated, providing additional lipid storage capacity. Finally, in proportion to lipid concentration, new rounds of LDs progressively assemble. Confocal microscopy and electron tomography suggest that emerging LDs are nucleated in a limited number of ER microdomains after a synchronized stepwise process of protein gathering, lipid packaging, and recognition by Plin3 and Plin2. A comparative analysis demonstrates that the acyl-CoA synthetase 3 is recruited early to the assembly sites, where it is required for efficient LD nucleation and lipid storage.
Matèries (anglès)
Citació
Col·leccions
Citació
KASSAN, Adam, HERMS FIOL, Albert, FERNÁNDEZ-VIDAL, Andrea, BOSCH I RODRÍGUEZ, Marta, SCHIEBER, Nicole l., REDDY, Babu j. n., FAJARDO, Alba, GELABERT BALDRICH, Mariona, TEBAR RAMON, Francesc, ENRICH BASTÚS, Carles, GROSS, Steven p., PARTON, Robert g., POL I SOROLLA, Albert. Acyl-CoA synthetase 3 promotes lipid droplet biogenesis in ER microdomains.. _Journal of Cell Biology_. 2013. Vol. 203, núm. 6, pàgs. 985-1001. [consulta: 20 de gener de 2026]. ISSN: 0021-9525. [Disponible a: https://hdl.handle.net/2445/54946]