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2019-02-18

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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/159622

Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells

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Recurs relacionat

https://doi.org/10.1038/s41563-019-0287-6

Resum

The generation of organoids is one of the biggest scientific advances in regenerative medicine. Here, by lengthening the time that human pluripotent stem cells (hPSCs) were exposed to a three-dimensional microenvironment, and by applying defined renal inductive signals, we generated kidney organoids that transcriptomically matched second-trimester human fetal kidneys. We validated these results using ex vivo and in vitro assays that model renal development. Furthermore, we developed a transplantation method that utilizes the chick chorioallantoic membrane. This approach created a soft in vivo microenvironment that promoted the growth and differentiation of implanted kidney organoids, as well as providing a vascular component. The stiffness of the in ovo chorioallantoic membrane microenvironment was recapitulated in vitro by fabricating compliant hydrogels. These biomaterials promoted the efficient generation of renal vesicles and nephron structures, demonstrating that a soft environment accelerates the differentiation of hPSC-derived kidney organoids.

Matèries

Medicina regenerativa, Cèl·lules mare

Matèries (anglès)

Regenerative medicine, Stem cells

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Articles publicats en revistes (Biomedicina)
Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
Articles publicats en revistes (Institut de Biomedicina (IBUB))

Citació

GARRETA, Elena, et al. Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells. Nature Materials. 2019. Vol. 18, num. 397-405. ISSN 1476-1122. [consulted: 23 of May of 2026]. Available at: https://hdl.handle.net/2445/159622

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