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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/130296
Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy
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Continuous cell culture monitoring as a way of investigating growth, proliferation,
and kinetics of biological experiments is in high demand. However, commercially available solutions
are typically expensive and large in size. Digital inline-holographic microscopes (DIHM) can provide
a cost-effective alternative to conventional microscopes, bridging the gap towards live-cell culture
imaging. In this work, a DIHM is built from inexpensive components and applied to different cell
cultures. The images are reconstructed by computational methods and the data are analyzed with
particle detection and tracking methods. Counting of cells as well as movement tracking of living
cells is demonstrated, showing the feasibility of using a field-portable DIHM for basic cell culture
investigation and bringing about the potential to deeply understand cell motility.
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SCHOLZ, Gregor, MARIANA, Shinta, DHARMAWAN, Agus budi, SYAMSU, Iqbal, HÖRMANN, Philipp, REUSE, Carsten, HARTMANN, Jana, HILLER, Karsten, PRADES GARCÍA, Juan daniel, WASISTO, Hutomo suryo, WAAG, Andreas. Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy. _Sensors_. 2019. Vol. 19, núm. 5, pàgs. 1234-1247. [consulta: 21 de gener de 2026]. ISSN: 1424-8220. [Disponible a: https://hdl.handle.net/2445/130296]