Document type
ArticleVersion
Published versionPublication date
Publication license
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/219524
Methyl-DNA immunoprecipitation (MeDIP): hunting down the DNA methylome
Journal Title
Director/Tutor
Journal ISSN
Volume Title
Related resource
Abstract
One of the most challenging projects in the field of epigenetics is the generation of detailed functional maps of DNA methylation in different cell and tissue types in normal and disease-associated conditions. This information will help us not only understand the role of DNA methylation but also identify targets for therapeutic treatment. The completion of the various epigenome projects depends on the design of novel strategies to survey and generate detailed cartograms of the DNA methylome. Methyl-DNA immunoprecipitation (MeDIP) assays, in combination with hybridization on high-resolution microarrays or high-throughput sequencing (HTS) techniques, are excellent methods for identifying methylated CpG-rich sequences. We provide a critical overview of different genome-wide techniques for DNA methylation analysis and propose that MeDIP assays may constitute a key method for elucidating the hypermethylome of cancer cells.
Subject
Subject (English)
Citation
Citation
JACINTO, Filipe V., BALLESTAR TARÍN, Esteban and ESTELLER, Manel. Methyl-DNA immunoprecipitation (MeDIP): hunting down the DNA methylome. Biotechniques. 2008. Vol. 44, num. 1, pags. 35-43. ISSN 0736-6205. [consulted: 8 of August of 2026]. Available at: https://hdl.handle.net/2445/219524