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2021

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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/193482

Monitoring emergence of SARS-CoV-2 B.1.1.7 Variant through the Spanish National SARSCoV-2 Wastewater Surveillance System (VATar COVID-19) from December 2020 to March 2021

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https://doi.org/10.1021/acs.est.1c03589

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Since its first identification in the United Kingdom in late 2020, the highly transmissible B.1.1.7 variant of SARS-CoV-2 has become dominant in several countries raising great concern. We developed a duplex real-time RT-qPCR assay to detect, discriminate, and quantitate SARS-CoV-2 variants containing one of its mutation signatures, the ΔHV69/70 deletion, and used it to trace the community circulation of the B.1.1.7 variant in Spain through the Spanish National SARS-CoV-2 Wastewater Surveillance System (VATar COVID-19). The B.1.1.7 variant was detected earlier than clinical epidemiological reporting by the local authorities, first in the southern city of Málaga (Andalucía) in week 20_52 (year_week), and multiple introductions during Christmas holidays were inferred in different parts of the country. Wastewater-based B.1.1.7 tracking showed a good correlation with clinical data and provided information at the local level. Data from wastewater treatment plants, which reached B.1.1.7 prevalences higher than 90% for ≥2 consecutive weeks showed that 8.1 ± 2.0 weeks were required for B.1.1.7 to become dominant. The study highlights the applicability of RT-qPCR-based strategies to track specific mutations of variants of concern as soon as they are identified by clinical sequencing and their integration into existing wastewater surveillance programs, as a cost-effective approach to complement clinical testing during the COVID-19 pandemic.

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SARS-CoV-2, COVID-19, Epidemiologia

Matèries (anglès)

SARS-CoV-2, COVID-19, Epidemiology

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Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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CARCERENY, Albert, et al. Monitoring emergence of SARS-CoV-2 B.1.1.7 Variant through the Spanish National SARSCoV-2 Wastewater Surveillance System (VATar COVID-19) from December 2020 to March 2021. Environmental Science & Technology. 2021. Vol. 55, num. 17, pags. 11756-11766. ISSN 0013-936X. [consulted: 27 of May of 2026]. Available at: https://hdl.handle.net/2445/193482

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