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2021

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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/193482

Monitoring emergence of SARS-CoV-2 B.1.1.7 Variant through the Spanish National SARSCoV-2 Wastewater Surveillance System (VATar COVID-19) from December 2020 to March 2021

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https://doi.org/10.1021/acs.est.1c03589

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Since its first identification in the United Kingdom in late 2020, the highly transmissible B.1.1.7 variant of SARS-CoV-2 has become dominant in several countries raising great concern. We developed a duplex real-time RT-qPCR assay to detect, discriminate, and quantitate SARS-CoV-2 variants containing one of its mutation signatures, the ΔHV69/70 deletion, and used it to trace the community circulation of the B.1.1.7 variant in Spain through the Spanish National SARS-CoV-2 Wastewater Surveillance System (VATar COVID-19). The B.1.1.7 variant was detected earlier than clinical epidemiological reporting by the local authorities, first in the southern city of Málaga (Andalucía) in week 20_52 (year_week), and multiple introductions during Christmas holidays were inferred in different parts of the country. Wastewater-based B.1.1.7 tracking showed a good correlation with clinical data and provided information at the local level. Data from wastewater treatment plants, which reached B.1.1.7 prevalences higher than 90% for ≥2 consecutive weeks showed that 8.1 ± 2.0 weeks were required for B.1.1.7 to become dominant. The study highlights the applicability of RT-qPCR-based strategies to track specific mutations of variants of concern as soon as they are identified by clinical sequencing and their integration into existing wastewater surveillance programs, as a cost-effective approach to complement clinical testing during the COVID-19 pandemic.

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SARS-CoV-2, COVID-19, Epidemiologia

Matèries (anglès)

SARS-CoV-2, COVID-19, Epidemiology

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Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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CARCERENY, Albert, MARTÍNEZ-VELÁZQUEZ, Adán, BOSCH, Albert, ALLENDE, Anna, TRUCHADO, Pilar, CASCALES, Jenifer, ROMALDE, Jesús, LOIS, Marta, POLO, David, SÁNCHEZ, Gloria, PÉREZ-CATALUÑA, Alba, DÍAZ-REOLID, Azahara, ANTÓN, Andrés, GREGOR, Josep, GARCIA-CEHIC, Damir, QUER, Josep, PALAU, Margarita, GONZÁLEZ RUANO, Cristina, PINTÓ SOLÉ, Rosa maría, GUIX, Susana. Monitoring emergence of SARS-CoV-2 B.1.1.7 Variant through the Spanish National SARSCoV-2 Wastewater Surveillance System (VATar COVID-19) from December 2020 to March 2021. _Environmental Science & Technology_. 2021. Vol. 55, núm. 17, pàgs. 11756-11766. [consulta: 24 de gener de 2026]. ISSN: 0013-936X. [Disponible a: https://hdl.handle.net/2445/193482]

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