Amb motiu del tancament d'estiu, la validació de documents es reprendrà a partir del 28 d'agost de 2026. Disculpeu les molèsties.
Con motivo del cierre de verano, la validación de documentos se reanudará a partir del 28 de agosto de 2026. Disculpad las molestias
Due to the summer closure, document validation will resume starting August 28, 2026. We apologize for any inconvenience.

Fitxers

172936.pdf (1.15 MB)

Tipus de document

Article

Versió

Versió publicada

Data de publicació

1999-09-17

Tots els drets reservats

Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/177488

p70 S6 kinase-mediated protein synthesis is a critical step for vascular endothelial cell proliferation

Títol de la revista

Autors

Director/Tutor

ISSN de la revista

Títol del volum

Recurs relacionat

https://doi.org/10.1074/jbc.274.38.26776

Resum

In this work, we analyzed the role of the PI3K-p70 S6 kinase (S6K) signaling cascade in the stimulation of endothelial cell proliferation. We found that inhibitors of the p42/p44 MAPK pathway (PD98059) and the PI3K-p70 S6K pathway (wortmannin, Ly294002, and rapamycin) all block thymidine incorporation stimulated by fetal calf serum in the resting mouse endothelial cell line 1G11. The action of rapamycin can be generalized, since it completely inhibits the mitogenic effect of fetal calf serum in primary endothelial cell cultures (human umbilical vein endothelial cells) and another established capillary endothelial cell line (LIBE cells). The inhibitory effect of rapamycin is only observed when the inhibitor is added at the early stages of G(0)-G(1) progression, suggesting an inhibitory action early in G(1). Rapamycin completely inhibits growth factor stimulation of protein synthesis, which perfectly correlates with the inhibition of cell proliferation. In accordance with its inhibitory action on protein synthesis, activation of cyclin D1 and p21 proteins by growth factors is also blocked by preincubation with rapamycin. Expression of a p70 S6K mutant partially resistant to rapamycin reverses the inhibitory effect of the drug on DNA synthesis, indicating that rapamycin action is via p70 S6K. Thus, in vascular endothelial cells, activation of protein synthesis via p70 S6K is an essential step for cell cycle progression in response to growth factors.

Matèries

Endoteli, Biosíntesi, Proteïnes quinases

Matèries (anglès)

Endothelium, Biosynthesis, Protein kinases

Citació

Col·leccions

Articles publicats en revistes (Ciències Fisiològiques)

Citació

VIÑALS CANALS, Francesc, CHAMBARD, Jean Claude and POUYSSÉGUR, Jacques. p70 S6 kinase-mediated protein synthesis is a critical step for vascular endothelial cell proliferation. Journal of Biological Chemistry. 1999. Vol. 274, num. 38, pags. 26774-26782. ISSN 0021-9258. [consulted: 4 of August of 2026]. Available at: https://hdl.handle.net/2445/177488

Exportar metadades

JSON - METS

Compartir registre