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2020-06-26

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cc-by-nc-nd (c) Elsevier B.V., 2020
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/170728

A rapid and simple method for the determination of organic acids in proteolytic enzymes by capillary electrophoresis with indirect ultraviolet detection

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Recurs relacionat

https://doi.org/10.1016/j.microc.2020.105204

Resum

The use of organic acids (e.g. acetic acid and gluconic acid) as additives during protease production is regarded as one of the simplest alternatives to increase enzyme stability and activity in many industrial processes. However, no methods have been described for the determination of organic acids in proteases and their contents have not been established yet. In this work, a novel, rapid and simple method for the determination of organic acids in proteolytic enzymes by capillary electrophoresis (CE) with indirect ultraviolet (UV) detection has been developed. Under the optimized conditions, the method was validated in terms of linearity, limit of detection (LOD), limit of quantification (LOQ) and intra-day and inter-day repeatability. Later, a sample pretreatment based on a hydroalcoholic microextraction was carefully optimized to obtain good recovery and repeatability and determine acetic and gluconic acids in a commercial protease sample. The complete procedure was validated using the standard-addition calibration method, finding matrix effects on the studied compounds. Finally, acetic acid and gluconic acid were quantified at 80 mg/Kg (0.0080% (m/m)) and 69 mg/Kg (0.0069% (m/m)) in the protease sample, respectively.

Matèries

Electroforesi capil·lar, Àcids carboxílics

Matèries (anglès)

Capillary electrophoresis, Carboxylic acids

Citació

Col·leccions

Articles publicats en revistes (Enginyeria Química i Química Analítica)

Citació

PONT VILLANUEVA, Laura, BARBOSA TORRALBO, José and BENAVENTE MORENO, Fernando J. (Julián). A rapid and simple method for the determination of organic acids in proteolytic enzymes by capillary electrophoresis with indirect ultraviolet detection. Microchemical Journal. 2020. Vol. 158, num. 105204-105210. ISSN 0026-265X. [consulted: 8 of August of 2026]. Available at: https://hdl.handle.net/2445/170728

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