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cc-by-nc (c) Aguilar, Mauricio R. et al., 2024
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/222886

Single-Molecule Electrical Conductance in Z-form DNA:RNA

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Nucleic acids have emerged as new materials with promising applications in nanotechnology, molecular electronics, and biosensing, but their electronic properties, especially at the single-molecule level, are largely underexplored. The Z-form is an exotic left-handed helical oligonucleotide conformation that may be involved in critical biological processes such as the regulation of gene expression and epigenetic processes. In this work, the electrical conductance of individual Guanine Cytosine (GC)-rich DNA:RNA molecules is measured in physiological buffer and 2,2,2-Trifluoroethanol (TFE) solvent, corresponding to the natural (right-handed helix) A-form typical in DNA:RNA hybrids and the (left-handed) Z-form conformations, respectively. Single-molecule conductance measurements are performed using the Scanning Tunneling Microscopy (STM)-assisted break-junction method in the so-called “blinking” approach, recording the spontaneous formation of single-biomolecule junctions and performing statistical analysis of the signals. Circular Dichroism (CD) experiments and ab initio calculations are also done to rationalize the measured molecular conductivity with a simple structural and electronic model. These results show that the electrical conductivity of the Z-form is one order of magnitude lower than that of the more compact A-form. The longer molecular length and higher energy for the Highest Occupied Molecular Orbital (HOMO) of the Z-form account for the differences in single-molecule conductance observed experimentally.

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AGUILAR, Mauricio r., JOVER MODREGO, Jesús, RUIZ SABÍN, Eliseo, ARAGONÈS, Albert c., ARTÉS, Juan m.. Single-Molecule Electrical Conductance in Z-form DNA:RNA. _Small_. 2024. [consulta: 24 de novembre de 2025]. ISSN: 1613-6810. [Disponible a: https://hdl.handle.net/2445/222886]

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