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2020-12-13

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cc-by (c) Fernández Cassi, Xavier et al., 2020
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/176426

Unveiling Viruses Associated with Gastroenteritis Using a Metagenomics Approach

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Recurs relacionat

https://doi.org/10.3390/v12121432

Resum

Acute infectious gastroenteritis is an important illness worldwide, especially on children, with viruses accounting for approximately 70% of the acute cases. A high number of these cases have an unknown etiological agent and the rise of next generation sequencing technologies has opened new opportunities for viral pathogen detection and discovery. Viral metagenomics in routine clinical settings has the potential to identify unexpected or novel variants of viral pathogens that cause gastroenteritis. In this study, 124 samples from acute gastroenteritis patients from 2012-2014 previously tested negative for common gastroenteritis pathogens were pooled by age and analyzed by next generation sequencing (NGS) to elucidate unidentified viral infections. The most abundant sequences detected potentially associated to acute gastroenteritis were from Astroviridae and Caliciviridae families, with the detection of norovirus GIV and sapoviruses. Lower number of contigs associated to rotaviruses were detected. As expected, other viruses that may be associated to gastroenteritis but also produce persistent infections in the gut were identified including several Picornaviridae members (EV, parechoviruses, cardioviruses) and adenoviruses. According to the sequencing data, astroviruses, sapoviruses and NoV GIV should be added to the list of viral pathogens screened in routine clinical analysis.

Matèries

Gastroenteritis, Infants, Metagenòmica

Matèries (anglès)

Gastroenteritis, Children, Metagenomics

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Col·leccions

Articles publicats en revistes (Genètica, Microbiologia i Estadística)

Citació

FERNÁNDEZ CASSI, Xavier, et al. Unveiling Viruses Associated with Gastroenteritis Using a Metagenomics Approach. Viruses. 2020. Vol. 12, num. 12. ISSN 1999-4915. [consulted: 3 of August of 2026]. Available at: https://hdl.handle.net/2445/176426

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