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2022-05-31

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cc-by (c) Pascual Benito, Miriam et al., 2022
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/200653

Comparison of methods for the enumeration of coliphages in 100 mL water samples

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https://doi.org/10.1016/j.scitotenv.2022.156381

Resum

In the last decade coliphages have been included in many water quality regulations as viral faecal indicators. However, the standardised methods used to detect and quantify coliphages differ in bacterial host strains, culture media and techniques. In this comparative study, 100 mL samples of mineral drinking water, river water and wastewater were analysed with International Organization for Standardization (ISO) standard methods, with United States-Environmental Protection Agency (U.S. EPA) based methods as well as commercial kits combining a single agar layer (SAL) assay with ISO bacterial host strains. The three methods gave similar counts (p-value>0.05) for somatic and total coliphages in the matrices with less than 100 PFU/100 mL, whereas for F-specific coliphages, the U.S. EPA method provided statistically significant lower numbers (p-value<0.05) than the other two protocols, possibly because it uses a different bacterial host strain (Escherichia coli HS (pFamp) R vs. the ISO strain Salmonella enterica serovar Typhimurium WG49). In samples with more than 100 PFU/100 mL, the ISO method yielded higher counts of somatic coliphages than the other two protocols (p-value<0.05). As the three methods provided similar results in clean water, the approach combining a SAL assay with the ISO bacterial host strain could be a useful option for coliphage analysis in this type of sample, as it does not require a concentration step.

Matèries

Qualitat de l'aigua, Bacteriòfags

Matèries (anglès)

Water quality, Bacteriophages

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Articles publicats en revistes (Genètica, Microbiologia i Estadística)

Citació

PASCUAL BENITO, Miriam, et al. Comparison of methods for the enumeration of coliphages in 100 mL water samples. Science of the Total Environment. 2022. Vol. 838, num. 156381. ISSN 0048-9697. [consulted: 3 of August of 2026]. Available at: https://hdl.handle.net/2445/200653

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