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Condensation Transition in DNA-Polyaminoamide Dendrimer Fibers Studied Using Optical Tweezers

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When mixed together, DNA and polyaminoamide dendrimers form fibers that condense into a compact structure. We use optical tweezers to pull condensed fibers and investigate the decondensation transition by measuring force-extension curves (FECs). A characteristic force plateau (around 10 pN) and hysteresis between the pulling and relaxation cycles are observed for different dendrimer sizes, indicating the existence of a first-order transition between two phases (condensed and extended) of the fiber. Upon salt variation FECs change noticeably confirming that electrostatic forces drive the condensation transition. We propose a simple model for the decondensing transition that qualitatively reproduces the FECs and which is confirmed by atomic force microscopy images.

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RITORT FARRAN, Fèlix, MIHARDJA, S., SMITH, S.b., BUSTAMANTE, C.j.. Condensation Transition in DNA-Polyaminoamide Dendrimer Fibers Studied Using Optical Tweezers. _Physical Review Letters_. 2006. Vol. 96, núm. 11, pàgs. 118301. [consulta: 20 de gener de 2026]. ISSN: 0031-9007. [Disponible a: https://hdl.handle.net/2445/134787]

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