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2009

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cc-by (c) Risson, Valérie et al., 2009
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/190447

Muscle inactivation of mTOR causes metabolic and dystrophin defects leading to severe myopathy

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https://doi.org/10.1083/jcb.200903131

Resum

Mammalian target of rapamycin (mTOR) is a key regulator of cell growth that associates with raptor and rictor to form the mTOR complex 1 (mTORC1) and mTORC2, respectively. Raptor is required for oxidative muscle integrity, whereas rictor is dispensable. In this study, we show that muscle-specific inactivation of mTOR leads to severe myopathy, resulting in premature death. mTOR-deficient muscles display metabolic changes similar to those observed in muscles lacking raptor, including impaired oxidative metabolism, altered mitochondrial regulation, and glycogen accumulation associated with protein kinase B/Akt hyperactivation. In addition, mTOR-deficient muscles exhibit increased basal glucose uptake, whereas whole body glucose homeostasis is essentially maintained. Importantly, loss of mTOR exacerbates the myopathic features in both slow oxidative and fast glycolytic muscles. Moreover, mTOR but not raptor and rictor deficiency leads to reduced muscle dystrophin content. We provide evidence that mTOR controls dystrophin transcription in a cell-autonomous, rapamycin-resistant, and kinase-independent manner. Collectively, our results demonstrate that mTOR acts mainly via mTORC1, whereas regulation of dystrophin is raptor and rictor independent.

Matèries

Proteïnes portadores, Malalties musculars, Animals, Glucosa

Matèries (anglès)

Carrier proteins, Muscular Diseases, Animals, Glucose

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Articles publicats en revistes (Ciències Fisiològiques)

Citació

RISSON, Valérie, MAZELIN, Laetitia, ROCERI, Mila, SANCHEZ, Hervé, MONCOLLIN, Vincent, CORNELOUP, Claudine, RICHARD-BULTEAU, Hélène, VIGNAUD, Alban, BAAS, Dominique, DEFOUR, Aurélia, FREYSSENET, Damien, TANTI, Jean-françois, LE-MARCHAND-BRUSTEL, Yannick, Ferrier. Bernard, CONJARD-DUPLANY, Agnès, ROMANINO, Klaas, BAUCHÉ, Stéphanie, HANTAÏ, Daniel, MUELLER, Matthias, KOZMA, Sara c, THOMAS, George, RÜEGG, Markus a., FERRY, Arnaud, PENDE, Mario, BIGARD, Xavier, KOULMANN, Nathalie, SCHAEFFER, Laurent, GANGLOFF, Yann-gael. Muscle inactivation of mTOR causes metabolic and dystrophin defects leading to severe myopathy. _Journal of Cell Biology_. 2009. Vol. 187, núm. 6, pàgs. 859-874. [consulta: 29 de gener de 2026]. ISSN: 0021-9525. [Disponible a: https://hdl.handle.net/2445/190447]

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