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2017

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cc-by-nc (c) Huguet, J. M. et al., 2017
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/125475

Derivation of nearest-neighbor DNA parameters in magnesium from single-molecule experiments

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Recurs relacionat

https://doi.org/10.1093/nar/gkx1161

Resum

DNA hybridization is an essential molecular reaction in biology with many applications. The nearest-neighbor (NN) model for nucleic acids predicts DNA thermodynamics using energy values for the different base pair motifs. These values have been derived from melting experiments in monovalent and divalent salt and applied to predict melting temperatures of oligos within a few degrees. However, an improved determination of the NN energy values and their salt dependencies in magnesium is still needed for current biotechnological applications seeking high selectivity in the hybridization of synthetic DNAs. We developed a methodology based on single molecule unzipping experiments to derive accurate NN energy values and initiation factors for DNA. A new set of values in magnesium is derived, which reproduces unzipping data and improves melting temperature predictions for all available oligo lengths, in a range of temperature and salt conditions where correlation effects between the magnesium bound ions are weak. The NN salt correction parameters are shown to correlate to the GC content of the NN motifs. Our study shows the power of single-molecule force spectroscopy assays to unravel novel features of nucleic acids such as sequence-dependent salt corrections.

Matèries

ADN, Cinètica química, Magnesi, Termodinàmica

Matèries (anglès)

DNA, Chemical kinetics, Magnesium, Thermodynamics

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Articles publicats en revistes (Física de la Matèria Condensada)
<b>Publicacions de projectes de recerca finançats per la UE</b>

Citació

HUGUET, J. m., RIBEZZI-CRIVELLARI, M., BIZARRO, C. v., RITORT FARRAN, Fèlix. Derivation of nearest-neighbor DNA parameters in magnesium from single-molecule experiments. _Nucleic Acids Research_. 2017. Vol. 45, núm. 22, pàgs. 12921-12931. [consulta: 23 de gener de 2026]. ISSN: 0305-1048. [Disponible a: https://hdl.handle.net/2445/125475]

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