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2023-08-01

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cc by-nc-dc (c) Vergara-Barberán, Maria et al., 2023
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/227976

On-line aptamer affinity solid-phase extraction capillary electrophoresis-mass spectrometry for the analysis of protein biomarkers in biological fluids and food: A tutorial

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https://doi.org/10.1016/j.sampre.2023.100082

Resum

The analysis by capillary electrophoresis (CE) of low abundant proteins in complex samples, such as biological fluids and food, is especially challenging, due to the poor concentration sensitivity of microscale separation tech- niques and the sample matrix complexity. In order to overcome these major drawbacks, microextraction sample preparation techniques based on on-line solid-phase extraction capillary electrophoresis (SPE-CE) are regarded as an excellent alternative for sample matrix clean-up and analyte preconcentration with minimum sample han- dling. In this study, we present, as a tutorial, a valve-free on-line aptamer affinity solid-phase extraction capillary electrophoresis-mass spectrometry (AA-SPE-CE-MS) method for purification, preconcentration, separation, de- tection, and characterization of intact protein biomarkers in biological fluids and food using as cases of study ‑synuclein ( ‑syn), concanavalin A (Con A), and -lactoglobulin ( -LG), which are related to Parkinson’s dis- ease and food allergy, respectively. This tutorial is not limited to the description of the analytical method, but it also provides ready-to-use preparation procedures for sorbent and microextraction devices, and introduces strategies to overcome undesired effects, allowing a straightforward implementation and optimum performance of AA-SPE-CE-MS, as a platform to develop further applications.

Matèries

Electroforesi capil·lar, Espectrometria de masses, Proteïnes

Matèries (anglès)

Capillary electrophoresis, Mass spectrometry, Proteins

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Articles publicats en revistes (Enginyeria Química i Química Analítica)
Articles publicats en revistes (Institut de Recerca en Nutrició i Seguretat Alimentària (INSA·UB))

Citació

VERGARA BARBERAN, Maria, et al. On-line aptamer affinity solid-phase extraction capillary electrophoresis-mass spectrometry for the analysis of protein biomarkers in biological fluids and food: A tutorial. Advances in Sample Preparation. 2023. Vol. 7, num. 100082. ISSN 2772-5820. [consulted: 22 of May of 2026]. Available at: https://hdl.handle.net/2445/227976

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