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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/165289
Assessment of Developmental Delay in the Zebrafish embryo Teratogenicity Assay
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In this study we analyzed some aspects of the assessment of developmental delay in the zebrafish embryotoxicity/teratogenicity test and explored the suitability of acetylcholinesterase (AChE) activity as a biochemical marker and as a higher throughput alternative to morphological endpoints such as head-trunk angle, tail length and morphological score. Embryos were exposed from 4 to 52 h post-fertilization (hpf) to a selection of known embryotoxic/teratogen compounds (valproic acid, retinoic acid, caffeine, sodium salicylate, glucose, hydroxyurea, methoxyacetic acid, boric acid and paraoxon-methyl) over a concentration range. They were evaluated for AChE activity, head-trunk angle, tail length and several qualitative parameters integrated in a morphological score. In general, the different patterns of the concentration-response curves allowed distinguishing between chemicals that produced growth retardation (valproic and methoxyacetic acid) and chemicals that produced non-growth-delay related malformations. An acceptable correlation between the morphological score, AChE activity and head-trunk angle as markers of developmental delay was observed, being AChE activity particularly sensitive to detect delay in the absence of malformations.
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TEIXIDÓ CONDOMINES, Elisabet, PIQUÉ BENAGES, Maria esther, GÓMEZ CATALÁN, Jesús, LLOBET MALLAFRÉ, Joan m. (joan maria). Assessment of Developmental Delay in the Zebrafish embryo Teratogenicity Assay. _Toxicology in Vitro_. 2013. Vol. 27, núm. 1, pàgs. 469-478. [consulta: 2 de desembre de 2025]. ISSN: 0887-2333. [Disponible a: https://hdl.handle.net/2445/165289]