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2024-05-27

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cc-by (c) Klingmann, F. et al., 2024
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/215944

Parallel illumination for depletion microscopy throughacousto-optic spatial light modulation

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Recurs relacionat

https://doi.org/https://doi.org/10.1051/jeos/2024031

Resum

State-of-the-art super-resolution microscopy techniques, including Stimulated Emission Depletion (STED), Reversible Saturable Optical Fluorescence Transitions (RESOLFT), and Switching Laser Mode (SLAM) microscopies, implement Laguerre-Gaussian beams, also known as vortex or doughnut beams to capture fluorescence information within a sub-wavelength area of the observed sample, effectively surpassing the diffraction limit and significantly improving the quality of the image. However, these techniques typically operate at point by point basis, involving time-consuming scanning of the sample to construct a complete, meaningful image. Therefore, for real-time live cell imaging purposes, the parallelization of illumination is crucial. In this study, we demonstrate the parallel generation of arbitrary arrays of Gaussian and Laguerre-Gaussian laser foci suitable for super-resolution microscopy. We achieve rapid scanning through the sample using acousto-optic spatial light modulation, a technique we have previously pioneered across various fields. By employing parallelized illumination with both Gaussian and doughnut beams, we aim to capture super-resolution images.

Matèries

Dispositius acustoòptics, Fluorescència, Microscòpia

Matèries (anglès)

Acoustooptical devices, Fluorescence, Microscopy

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Col·leccions

Articles publicats en revistes (Física Aplicada)
Articles publicats en revistes (Institut de Nanociència i Nanotecnologia (IN2UB))

Citació

KLINGMANN, Fabian, et al. Parallel illumination for depletion microscopy throughacousto-optic spatial light modulation. Journal of The European Optical Society-Rapid Publications. 2024. Vol. 20, num. 2. ISSN 1990-2573. [consulted: 4 of August of 2026]. Available at: https://hdl.handle.net/2445/215944

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