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2019-01

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Marinobacter maroccanus sp. nov., a moderately halophilic bacterium isolated from a saline soil.

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https://doi.org/10.1099/ijsem.0.003134

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During the taxonomic investigation of exopolymer producing halophilic bacteria, a rod shaped, motile, Gram-stain-negative, aerobic, halophilic bacterium, designated strain N4T, was isolated from a natural saline soil located in the northern Morocco. The optimal growth of the isolate was at 30-37 ºC and at pH 6.0-9.0, in the presence of 5-7% (w/v) NaCl. Useful tests for the phenotypic differentiation of strain N4T from other Marinobacter species included α-chymotrypsin and α-glucosidase activities and the carbohydrate assimilation profile. The major fatty acids detected in strain N4T were C18:1 ω9c, C16:0, and C16:1 ω7c/C15:0 iso 2-OH. Sequence analysis of the 16S rRNA indicated that strain N4T belonged to the genus Marinobacter and was closely related to Marinobacter adhaerens NC17506T (99.04%), Marinobacter salsuginis SD-14BT (98.97%), and Marinobacter flavimaris SW-145T (98.36%). Phylogenetic analysis of the rpoD gene sequence also showed that the nearest neighbours of strain N4T were M. adhaerens (90.63%) and M. salsuginis (91.13%). Strain N4T showed 87.98% similarity in the average nucleotide identity (ANI) with M. flavimaris and M. salsuginis, and 87.47% with M. adhaerens. In the in-silico genome-to-genome distance (GGD), strain N4T showed DNA-DNA hybridization (DDH) values of 33.30% with M. adhaerens, 34.60% with M. flavimaris and 34.70% with M. salsuginis. DNA G+C content of N4T was 57.3 mol%. Based on the results of phenotypic characterization, phylogenetic analysis and genome comparison, strain N4T represents a novel species of the genus Marinobacter, for which the name Marinobacter maroccanus sp. nov. is proposed. The type strain is N4T (=CECT 9525T=LMG 30466T).

Matèries

Genètica bacteriana, Filogènia, Taxonomia (Biologia), Microbiologia dels sòls, Marroc

Matèries (anglès)

Bacterial genetics, Phylogeny, Taxonomy (Biology), Soil microbiology, Morocco

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Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)

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BOUJIDA, Nadia, PALAU, Montserrat, CHARFI, Saoulajan, MANRESA PRESAS, Ma. ángeles (maría ángeles), SKALI SENHAJI, Nadia, ABRINI, Jamal, MIÑANA I GALBIS, David. Marinobacter maroccanus sp. nov., a moderately halophilic bacterium isolated from a saline soil.. _International Journal of Systematic and Evolutionary Microbiology_. 2019. Vol. 69, núm. 1, pàgs. 227-234. [consulta: 29 de gener de 2026]. ISSN: 1466-5026. [Disponible a: https://hdl.handle.net/2445/165145]

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