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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/192166
Rich dynamics and functional organization on topographically designed neuronal networks in vitro
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Neuronal cultures are a prominent experimental tool to understand complex functional organization in neuronal assemblies. However, neurons grown on flat surfaces exhibit a strongly coherent bursting behavior with limited functionality. To approach the functional richness of naturally formed neuronal circuits, here we studied neuronal networks grown on polydimethylsiloxane (PDMS) topographical patterns shaped as either parallel tracks or square valleys.We followed the evolution of spontaneous activity in these cultures along 20 days in vitro using fluorescence calcium imaging. The networks were characterized by rich spatiotemporal activity patterns that comprised from small regions of the culture to its whole extent. Effective connectivity analysis revealed the emergence of spatially compact functional modules that were associated with both the underpinned topographical features and predominant spatiotemporal activity fronts. Our results showthe capacity of spatial constraints tomold activity and functional organization, bringing new opportunities to comprehend the structure-function relationship in living neuronal circuits.
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MONTALÀ FLAQUER, Marc, et al. Rich dynamics and functional organization on topographically designed neuronal networks in vitro. iScience. 2022. Vol. 25, num. 12, pags. 105680. ISSN 2589-0042. [consulted: 15 of June of 2026]. Available at: https://hdl.handle.net/2445/192166