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2019-03-01

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cc-by (c) Teixidó Condomines, Elisabet et al., 2019
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/165764

Cardiovascular effects of PCB 126 (3,3',4,4',5-pentachlorobiphenyl) in zebrafish embryos and impact of co-exposure to redox modulating chemicals

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https://doi.org/10.3390/ijms20051065

Abstract

The developing cardiovascular system of zebrafish is a sensitive target for many environmental pollutants, including dioxin-like compounds and pesticides. Some polychlorinated biphenyls (PCBs) can compromise the cardiovascular endothelial function by activating oxidative stress-sensitive signaling pathways. Therefore, we exposed zebrafish embryos to PCB126 or to several redox-modulating chemicals to study their ability to modulate the dysmorphogenesis produced by PCB126. PCB126 produced a concentration-dependent induction of pericardial edema and circulatory failure, and a concentration-dependent reduction of cardiac output and body length at 80 hours post fertilization (hpf). Among several modulators tested, the effects of PCB126 could be both positively and negatively modulated by different compounds; co-treatment with -tocopherol (vitamin E liposoluble) prevented the adverse effects of PCB126 in pericardial edema, whereas co-treatment with sodium nitroprusside (a vasodilator compound) significantly worsened PCB126 effects. Gene expression analysis showed an up-regulation of cyp1a, hsp70, and gstp1, indicative of PCB126 interaction with the aryl hydrocarbon receptor (AhR), while the transcription of antioxidant genes (sod1, sod2; cat and gpx1a) was not affected. Further studies are necessary to understand the role of oxidative stress in the developmental toxicity of low concentrations of PCB126 (25 nM). Our results give insights into the use of zebrafish embryos for exploring mechanisms underlying the oxidative potential of environmental pollutants.

Subject

Embriologia, Fenotip, Farmacologia, Toxicologia, Estrès oxidatiu, Peix zebra

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Embryology, Phenotype, Pharmacology, Toxicology, Oxidative stress, Zebra danio

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Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)

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TEIXIDÓ CONDOMINES, Elisabet, et al. Cardiovascular effects of PCB 126 (3,3',4,4',5-pentachlorobiphenyl) in zebrafish embryos and impact of co-exposure to redox modulating chemicals. International Journal of Molecular Sciences. 2019. Vol. 20, num. 5, pags. 1065. ISSN 1661-6596. [consulted: 11 of June of 2026]. Available at: https://hdl.handle.net/2445/165764

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