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2013-01

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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/36347

Three-step pathway engineering results in more incidence rate and higher emission of nerolidol and improved attraction of Diadegma semiclausum

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http://dx.doi.org/10.1016/j.ymben.2012.10.002

Resum

The concentration and ratio of terpenoids in the headspace volatile blend of plants have a fundamental role in the communication of plants and insects. The sesquiterpene (E)-nerolidol is one of the important volatiles with effect on beneficial carnivores for biologic pest management in the field. To optimize de novo biosynthesis and reliable and uniform emission of (E)-nerolidol, we engineered different steps of the (E)-nerolidol biosynthesis pathway in Arabidopsis thaliana. Introduction of a mitochondrial nerolidol synthase gene mediates de novo emission of (E)-nerolidol and linalool. Co-expression of the mitochondrial FPS1 and cytosolic HMGR1 increased the number of emitting transgenic plants (incidence rate) and the emission rate of both volatiles. No association between the emission rate of transgenic volatiles and their growth inhibitory effect could be established. (E)-Nerolidol was to a large extent metabolized to non-volatile conjugates.

Matèries

Àrabis, Terpens, Enginyeria genètica vegetal, Insectes paràsits, Mecanismes de defensa en les plantes

Matèries (anglès)

Arabis, Terpenes, Plant genetic engineering, Parasitic insects, Plant defenses

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Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

Citació

HOUSHYANI, Benyamin, ASSAREH, Maryam, BUSQUETS BISBAL, Antonio, FERRER I PRATS, Albert, BOUWMEESTER, Harro j., KAPPERS, Iris f.. Three-step pathway engineering results in more incidence rate and higher emission of nerolidol and improved attraction of Diadegma semiclausum. _Metabolic Engineering_. 2013. Vol. 15, núm. 88-97. [consulta: 26 de gener de 2026]. ISSN: 1096-7176. [Disponible a: https://hdl.handle.net/2445/36347]

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