Fibronectin fibrillogenesis influence on nuclear dynamics in polarized fibroblasts

Abstract

In this project we studied the influence of fibrillar adhesions formation on nuclear dynamics and cell migration in human fibroblasts. We first show that their formation is a migrationdependent process that is not observed when fibroblasts are confined. Then, we present significant evidence that in absence of fibrillar adhesions nuclei align with the long axis of the cell less effectively. Moreover, we present promising data that show that nuclear positioning and cell migration arealso altered in these conditions. In conclusion, we establish that fibrillar adhesions formation is a potentially important parameter of nuclear and cellular dynamics in migrating human fibroblasts