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2004-10

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cc-by-nc-nd (c) Matarazzo, M.R. et al., 2004
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/219522

In vivo analysis of DNA methylation patterns recognized by specific proteins: coupling CHIP and bisulfite analysis

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https://doi.org/10.2144/04374DD02

Abstract

The three-way connection between DNA methylation, chromatin configuration, and transcriptional regulation is under increasing attention, but the fine rules governing the epigenetic control are still poorly understood. In several studies, the authors have concluded that the methylation status of CpG sites could be critical for the binding of factors to DNA and, consequently, for chromatin conformation. We tested the possibility that a novel technical approach combining chromatin immunoprecipitation and bisulfite genomic sequencing analysis (ChIP-BA) could provide useful information on the role of specific CpG methylation patterns in driving the association in vivo of proteins to given genomic regions. Our results show that ChIP-BA permits the establishment in vivo of the methylation patterns required for the binding of a methyl-CpG binding protein and, in addition, can potentially identify methylation patterns that do not allow a protein to bind specific genomic regions. Possible fields of application are discussed. We believe that wide use of ChIP-BA could make possible the exploration of a novel aspect of the intricate epigenetic web.

Subject

Cromatina, ADN, Genòmica, Clonatge molecular

Subject (English)

Chromatin, DNA, Genomics, Molecular cloning

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Articles publicats en revistes (Ciències Fisiològiques)

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MATARAZZO, Maria Rosaria, et al. In vivo analysis of DNA methylation patterns recognized by specific proteins: coupling CHIP and bisulfite analysis. Biotechniques. 2004. Vol. 37, num. 4, pags. 666-673. ISSN 0736-6205. [consulted: 8 of August of 2026]. Available at: https://hdl.handle.net/2445/219522

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