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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/198245
Temperature-dependent elastic properties of DNA
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Knowledge of the elastic properties, e.g., the persistence length or interphosphate distance, of single-stranded (ss) and double-stranded (ds) DNA under different experimental conditions is critical to characterizing molecular reactions studied with single-molecule techniques. While previous experiments have addressed the dependence of the elastic parameters upon varying ionic strength and contour length, temperature-dependent effects are less studied. Here, we examine the temperature-dependent elasticity of ssDNA and dsDNA in the range 5°C-50°C using a temperature-jump optical trap. We find a temperature softening for dsDNA and a temperature stiffening for ssDNA. Our results highlight the need for a general theory explaining the phenomenology observed.
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RICO PASTÓ, Marc and RITORT FARRAN, Fèlix. Temperature-dependent elastic properties of DNA. Biophysical Reports. 2022. Vol. 2, num. 3, pags. 1067. ISSN 2667-0747. [consulted: 8 of August of 2026]. Available at: https://hdl.handle.net/2445/198245