Amb motiu del tancament d'estiu, la validació de documents es reprendrà a partir del 28 d'agost de 2026. Disculpeu les molèsties.
Con motivo del cierre de verano, la validación de documentos se reanudará a partir del 28 de agosto de 2026. Disculpad las molestias
Due to the summer closure, document validation will resume starting August 28, 2026. We apologize for any inconvenience.

Files

725103.pdf (4.68 MB)

Document type

Article

Version

Published version

Publication date

2022-03-01

Publication license

cc-by (c) López-Molina, Laura et al., 2022
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/195525

Pyk2 Regulates MAMs and Mitochondrial Dynamics in Hippocampal Neurons

Journal Title

Authors

Director/Tutor

Journal ISSN

Volume Title

Related resource

https://doi.org/10.3390/cells11050842

Abstract

Pyk2 is a non-receptor tyrosine kinase enriched in hippocampal neurons, which can be activated by calcium-dependent mechanisms. In neurons, Pyk2 is mostly localised in the cytosol and dendritic shafts but can translocate to spines and/or to the nucleus. Here, we explore the function of a new localisation of Pyk2 in mitochondria-associated membranes (MAMs), a subdomain of ER-mitochondria surface that acts as a signalling hub in calcium regulation. To test the role of Pyk2 in MAMs' calcium transport, we used full Pyk2 knockout mice (Pyk2-/-) for in vivo and in vitro studies. Here we report that Pyk2-/- hippocampal neurons present increased ER-mitochondrial contacts along with defective calcium homeostasis. We also show how the absence of Pyk2 modulates mitochondrial dynamics and morphology. Taken all together, our results point out that Pyk2 could be highly relevant in the modulation of ER-mitochondria calcium efflux, affecting in turn mitochondrial function.

Subject

Mitocondris, Dinàmica d'una partícula, Hipocamp (Cervell), Neurones, Neurobiologia molecular

Subject (English)

Mitochondria, Dynamics of a particle, Hippocampus (Brain), Neurons, Molecular neurobiology

Citation

Collections

Articles publicats en revistes (Biomedicina)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Institut de Neurociències (UBNeuro))

Citation

LÓPEZ MOLINA, Laura, et al. Pyk2 Regulates MAMs and Mitochondrial Dynamics in Hippocampal Neurons. Cells. 2022. Vol. 11, num. 5, pags. 842. ISSN 2073-4409. [consulted: 9 of August of 2026]. Available at: https://hdl.handle.net/2445/195525

Export metadata

JSON - METS

Share record