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cc-by (c) Bach Griera, Marc et al., 2020
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/174370

Mycolicibacterium brumae is a safe and non-toxic immunomodulatory agent for cancer treatment

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Intravesical Mycobacterium bovis Bacillus Calmette-Guérin (BCG) immunotherapy remains the gold-standard treatment for non-muscle-invasive bladder cancer patients, even though half of the patients develop adverse events to this therapy. On exploring BCG-alternative therapies, Mycolicibacterium brumae, a nontuberculous mycobacterium, has shown outstanding anti-tumor and immunomodulatory capabilities. As no infections due to M. brumae in humans, animals, or plants have been described, the safety and/or toxicity of this mycobacterium have not been previously addressed. In the present study, an analysis was made of M. brumae- and BCG-intravenously-infected severe combined immunodeficient (SCID) mice, M. brumae-intravesically-treated BALB/c mice, and intrahemacoelic-infected-Galleria mellonella larvae. Organs from infected mice and the hemolymph from larvae were processed to count bacterial burden. Blood samples from mice were also taken, and a wide range of hematological and biochemical parameters were analyzed. Finally, histopathological alterations in mouse tissues were evaluated. Our results demonstrate the safety and non-toxic profile of M. brumae. Di erences were observed in the biochemical, hematological and histopathological analysis between M. brumae and BCG-infected mice, as well as survival curves rates and colony forming units (CFU) counts in both animal models. M. brumae constitutes a safe therapeutic biological agent, overcoming the safety and toxicity disadvantages presented by BCG in both mice and G. mellonella animal models.

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BACH GRIERA, Marc, CAMPO PÉREZ, Víctor, BARBOSA, Sandra, TRASERRA, Sara, GUALLAR GARRIDO, Sandra, MOYA ANDÉRICO, Laura, HERRERO ABADÍA, Paula, LUQUIN, Marina, RABANAL PRADOS, Rosa ma. (rosa maria), TORRENTS SERRA, Eduard, JULIÁN, Esther. Mycolicibacterium brumae is a safe and non-toxic immunomodulatory agent for cancer treatment. _Vaccines_. 2020. Vol. 8(2), núm. 198. [consulta: 7 de febrer de 2026]. ISSN: 2076-393X. [Disponible a: https://hdl.handle.net/2445/174370]

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