Amb motiu del tancament d'estiu, la validació de documents es reprendrà a partir del 28 d'agost de 2026. Disculpeu les molèsties.
Con motivo del cierre de verano, la validación de documentos se reanudará a partir del 28 de agosto de 2026. Disculpad las molestias
Due to the summer closure, document validation will resume starting August 28, 2026. We apologize for any inconvenience.

Fitxers

207201.pdf (2.66 MB)

Tipus de document

Article

Versió

Versió publicada

Data de publicació

2017-03-02

Llicència de publicació

cc-by (c) Alemany, Anna, et al., 2017
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/214309

Force-Dependent Folding and Unfolding Kinetics in DNA Hairpins Reveals Transition-State Displacements along a Single Pathway

Títol de la revista

Autors

Director/Tutor

ISSN de la revista

Títol del volum

Recurs relacionat

https://doi.org/10.1021/acs.jpclett.6b02687

Resum

Biomolecules diffusively explore their energy landscape overcoming energy barriers via thermally activated processes to reach the biologically relevant conformation. Mechanically induced unfolding and folding reactions offer an excellent playground to feature these processes at the single-molecule level by monitoring changes in the molecular extension. Here we investigate two-state DNA hairpins designed to have the transition states at different locations. We use optical 8 tweezers to characterize the force-dependent behavior of the kinetic barrier from nonequilibrium pulling experiments by using the continuous effective barrier approach (CEBA). We introduce the mechanical fragility and the molecular transition-state susceptibility, both useful quantities to characterize the response of the transition state to an applied force. Our results demonstrate the validity of the Leffler Hammond postulate where the transition state approaches the folded state as force increases, implying monotonically decreasing fragility with force and a non negative transition state susceptibility at all forces.

Matèries

Genètica, Molècules, Energia

Matèries (anglès)

Genetics, Molecules, Energy

Citació

Col·leccions

Articles publicats en revistes (Física de la Matèria Condensada)

Citació

ALEMANY, Anna, RITORT FARRAN, Fèlix and RICO PASTÓ, Marc. Force-Dependent Folding and Unfolding Kinetics in DNA Hairpins Reveals Transition-State Displacements along a Single Pathway. Journal of Physical Chemistry Letters. 2017. Vol. 8, num. 5, pags. 895-900. ISSN 1948-7185. [consulted: 19 of August of 2026]. Available at: https://hdl.handle.net/2445/214309

Exportar metadades

JSON - METS

Compartir registre