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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/214309
Force-Dependent Folding and Unfolding Kinetics in DNA Hairpins Reveals Transition-State Displacements along a Single Pathway
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Biomolecules diffusively explore their energy landscape overcoming energy barriers via thermally activated processes to reach the biologically relevant conformation. Mechanically induced unfolding and folding reactions offer an excellent playground to feature these processes at the single-molecule level by monitoring changes in the molecular extension. Here we investigate two-state DNA hairpins designed to have the transition states at different locations. We use optical 8 tweezers to characterize the force-dependent behavior of the kinetic barrier from nonequilibrium pulling experiments by using the continuous effective barrier approach (CEBA). We introduce the mechanical fragility and the molecular transition-state susceptibility, both useful quantities to characterize the response of the transition state to an applied force. Our results demonstrate the validity of the Leffler Hammond postulate where the transition state approaches the folded state as force increases, implying monotonically decreasing fragility with force and a non negative transition state susceptibility at all forces.
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ALEMANY, Anna, RITORT FARRAN, Fèlix and RICO PASTÓ, Marc. Force-Dependent Folding and Unfolding Kinetics in DNA Hairpins Reveals Transition-State Displacements along a Single Pathway. Journal of Physical Chemistry Letters. 2017. Vol. 8, num. 5, pags. 895-900. ISSN 1948-7185. [consulted: 22 of May of 2026]. Available at: https://hdl.handle.net/2445/214309