Amb motiu del tancament d'estiu, la validació de documents es reprendrà a partir del 28 d'agost de 2026. Disculpeu les molèsties.
Con motivo del cierre de verano, la validación de documentos se reanudará a partir del 28 de agosto de 2026. Disculpad las molestias
Due to the summer closure, document validation will resume starting August 28, 2026. We apologize for any inconvenience.

Files

036688.pdf (1.5 MB)

Document type

Article

Version

Published version

Publication date

1989-01-01

All rights reserved

Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/34020

Botulinum neurotoxin type A blocks the morphological changes induced by chemical stimulation on the presynaptic membrane of Torpedo synaptosomes.

Journal Title

Authors

Director/Tutor

Journal ISSN

Volume Title

Related resource

Abstract

The action of botulinum neurotoxin on acetylcholine release, and on the structural changes at the presynaptic membrane associated with the transmitter release,was studied by using a subcellular fraction of cholinergic nerve terminals (synaptosomes) isolated from the Torpedo electric organ. Acetylcholine and ATP release were continuously monitored by chemiluminescent methods.To catch the membrane morphological changes, the quick-freezing method was applied. Our results show that botulinum neurotoxin inhibits the release of acetylcholine from these isolated nerve terminals in a dose-dependent manner, whereas ATP release is not affected. The maximal inhibition (70%) is achieved at neurotoxin concentrations as low as 125 pM with an incubation time of 6 min. This effect is not linked to an alteration of the integrity of the synaptosomes since, after poisoning by botulinum neurotoxin type A, they show a nonmodified occluded lactate dehydrogenase activity. Moreover, membrane potential is not altered by the toxin with respect to the control, either in resting condition or after potassium depolarization. In addition to acetylcholine release inhibition, botulinum neurotoxin blocks the rearrangement of the presynaptic intramembrane particles induced by potassium stimulation. The action of botulinum neurotoxin suggests that the intramembrane particle rearrangement is related to the acetylcholine secretion induced by potassium stimulation in synaptosomes isolated from the electric organ of Torpedo marmorata.

Subject

Toxina botulínica, Sinapsi, Neurotransmissió

Subject (English)

Botulinum toxin, Synapses, Neural transmission

Citation

Collections

Articles publicats en revistes (Biomedicina)

Citation

MARSAL TEBÉ, Jordi, et al. Botulinum neurotoxin type A blocks the morphological changes induced by chemical stimulation on the presynaptic membrane of Torpedo synaptosomes. Proceedings of the National Academy of Sciences of the United States of America - PNAS. 1989. Vol. 86, num. 1, pags. 372-376. ISSN 0027-8424. [consulted: 18 of August of 2026]. Available at: https://hdl.handle.net/2445/34020

Export metadata

JSON - METS

Share record