Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Tots els drets reservats
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/122383
Actin-binding protein α-actinin-1 interacts with the metabotropic glutamate receptor type 5b and modulates the cell surface expression and function of the receptor
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
Receptors for neurotransmitters require scaffolding proteins for membrane microdomain targeting and for regulating receptor function. Using a yeast two-hybrid screen, α-actinin-1, a major F-actin cross-linking protein, was identified as a binding partner for the C-terminal domain of metabotropic glutamate receptor type 5b (mGlu5b receptor). Co-expression, co-immunoprecipitation, and pull-down experiments showed a close and specific interaction between mGlu5b receptor and α-actinin-1 in both transfected HEK-293 cells and rat striatum. The interaction of α-actinin-1 with mGlu5b receptor modulated the cell surface expression of the receptor. This was dependent on the binding of α-actinin-1 to the actin cytoskeleton. In addition, the α-actinin-1/mGlu5b receptor interaction regulated receptor-mediated activation of the mitogen-activated protein kinase pathway. Together, these findings indicate that there is an α-actinin-1-dependent mGlu5b receptor association with the actin cytoskeleton modulating receptor cell surface expression and functioning.
Matèries
Matèries (anglès)
Citació
Citació
CABELLO, Nuria, REMELLI, Rosaria, CANELA, Laia, SORIGUERA, Ana, MALLOL MONTERO, Josefa, CANELA CAMPOS, Enric i. (enric isidre), ROBBINS, Melanie j., LLUÍS I BISET, Carme, FRANCO FERNÁNDEZ, Rafael, MCILHINNEY, R. a. jeffrey, CIRUELA ALFÉREZ, Francisco. Actin-binding protein α-actinin-1 interacts with the metabotropic glutamate receptor type 5b and modulates the cell surface expression and function of the receptor. _Journal of Biological Chemistry_. 2007. Vol. 282, núm. 16, pàgs. 12143-12153. [consulta: 22 de febrer de 2026]. ISSN: 0021-9258. [Disponible a: https://hdl.handle.net/2445/122383]