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2021-01-12

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cc-by (c) Fuentes Pardo, Cristina et al., 2021
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/174950

Inactivation of hepatitis A virus and human norovirus in clams subjected to heat treatment

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Recurs relacionat

https://doi.org/10.3389/fmicb.2020.578328

Resum

Bivalve mollusk contamination by enteric viruses, especially human noroviruses (HuNoV) and hepatitis A virus (HAV), is a problem with health and economic implications. The aim of the study was the evaluation of the effect of heat treatment in clams (Tawera gayi) experimentally contaminated with HuNoV using a PMA-viability RTqPCR assay to minimize measurement of non-infectious viruses, and used HAV as a model to estimate infectivity loss. Spiked clams were immersed in water at 90°C to ensure that internal meat temperature was maintained above 90°C for at least 5 min. The treatment resulted in >3.89 ± 0.24 log10 TCID50/g reduction of infectious HAV, confirming inactivation. For HuNoV, RTqPCR assays showed log10 reductions of 2.96 ± 0.79 and 2.56 ± 0.56, for GI and GII, respectively, and the use of PMA resulted in an additional log10 reduction for GII, providing a better correlation with risk reduction. In the absence of a cell culture system which could be used to determine HuNoV infectivity reduction, a performance criteria based on PMA-RTqPCR log reduction could be used to evaluate food product safety. According to data from this study, heat treatments of clams which cause reductions >3.5 log10 for GII as measured by PMA-RTqPCR assay may be regarded as an acceptable inactivation treatment, and could be set as a performance criterion to test the effectiveness of other time-temperature inactivation processes.

Matèries

Hepatitis A, Terapèutica

Matèries (anglès)

Hepatitis A, Therapeutics

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Articles publicats en revistes (Genètica, Microbiologia i Estadística)
Publicacions de projectes de recerca finançats per la UE

Citació

FUENTES PARDO, Cristina, et al. Inactivation of hepatitis A virus and human norovirus in clams subjected to heat treatment. Frontiers in Microbiology. 2021. Vol. 11, num. 578328. ISSN 1664-302X. [consulted: 12 of August of 2026]. Available at: https://hdl.handle.net/2445/174950

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