Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/174950
Inactivation of hepatitis A virus and human norovirus in clams subjected to heat treatment
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
Bivalve mollusk contamination by enteric viruses, especially human noroviruses (HuNoV) and hepatitis A virus (HAV), is a problem with health and economic implications. The aim of the study was the evaluation of the effect of heat treatment in clams (Tawera gayi) experimentally contaminated with HuNoV using a PMA-viability RTqPCR assay to minimize measurement of non-infectious viruses, and used HAV as a model to estimate infectivity loss. Spiked clams were immersed in water at 90°C to ensure that internal meat temperature was maintained above 90°C for at least 5 min. The treatment resulted in >3.89 ± 0.24 log10 TCID50/g reduction of infectious HAV, confirming inactivation. For HuNoV, RTqPCR assays showed log10 reductions of 2.96 ± 0.79 and 2.56 ± 0.56, for GI and GII, respectively, and the use of PMA resulted in an additional log10 reduction for GII, providing a better correlation with risk reduction. In the absence of a cell culture system which could be used to determine HuNoV infectivity reduction, a performance criteria based on PMA-RTqPCR log reduction could be used to evaluate food product safety. According to data from this study, heat treatments of clams which cause reductions >3.5 log10 for GII as measured by PMA-RTqPCR assay may be regarded as an acceptable inactivation treatment, and could be set as a performance criterion to test the effectiveness of other time-temperature inactivation processes.
Matèries
Matèries (anglès)
Citació
Citació
FUENTES PARDO, Cristina, PÉREZ-RODRÍGUEZ, Francisco javier, SABRIÀ SUNYÉ, Aurora, BEGUIRISTAIN CELAYETA, Nerea, PINTÓ SOLÉ, Rosa maría, GUIX ARNAU, Susana, BOSCH, Albert. Inactivation of hepatitis A virus and human norovirus in clams subjected to heat treatment. _Frontiers in Microbiology_. 2021. Vol. 11, núm. 578328. [consulta: 25 de febrer de 2026]. ISSN: 1664-302X. [Disponible a: https://hdl.handle.net/2445/174950]