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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/119377
miRNA let-7e modulates the wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation
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This study indicates that embryonic stem cells [ESCs] cultured with retinoic acid and activin A significantly upregulate the miRNA let-7e. This specific miRNA modulates the Wnt pathway and the expression of early nephrogenic markers under these differentiation conditions. The differentiation markers WT1, Pax2 and Wnt4 were downregulated when miRNA let-7e was silenced, thus indicating the role of miRNA let-7e in the differentiation process. PKCβ, GSK3β phosphorylation (GSK3β(P)) and β-catenin expression was reduced in differentiated cells and reversed by miRNA let-7e silencing. Addition of a PKCβ inhibitor to the miRNA let-7e silenced cells abolished let-7e-derived effects in differentiation markers, and reversed the increase in GSK3β(P) and β-catenin, thus indicating that miRNA let-7e is involved in differentiation via the modulation of GSK3β phosphorylation and β-catenin production.
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VIÑAS MUÑOZ, José luis, VENTAYOL ESPINAZO, Marina, BRÜNE, Bernhard, JUNG, Michaela, SOLA MARTÍNEZ, Anna, PI I SIQUÉS, Felip, MASTORA, Chrysoula, HOTTER CORRIPIO, Georgina. miRNA let-7e modulates the wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation. _PLoS One_. 2013. Vol. 8, núm. 4, pàgs. e60937. [consulta: 27 de gener de 2026]. ISSN: 1932-6203. [Disponible a: https://hdl.handle.net/2445/119377]