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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/119377
miRNA let-7e modulates the wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation
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This study indicates that embryonic stem cells [ESCs] cultured with retinoic acid and activin A significantly upregulate the miRNA let-7e. This specific miRNA modulates the Wnt pathway and the expression of early nephrogenic markers under these differentiation conditions. The differentiation markers WT1, Pax2 and Wnt4 were downregulated when miRNA let-7e was silenced, thus indicating the role of miRNA let-7e in the differentiation process. PKCβ, GSK3β phosphorylation (GSK3β(P)) and β-catenin expression was reduced in differentiated cells and reversed by miRNA let-7e silencing. Addition of a PKCβ inhibitor to the miRNA let-7e silenced cells abolished let-7e-derived effects in differentiation markers, and reversed the increase in GSK3β(P) and β-catenin, thus indicating that miRNA let-7e is involved in differentiation via the modulation of GSK3β phosphorylation and β-catenin production.
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VIÑAS MUÑOZ, José Luis, et al. miRNA let-7e modulates the wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation. PLoS One. 2013. Vol. 8, num. 4, pags. e60937. ISSN 1932-6203. [consulted: 7 of August of 2026]. Available at: https://hdl.handle.net/2445/119377