Amb motiu del tancament d'estiu, la validació de documents es reprendrà a partir del 28 d'agost de 2026. Disculpeu les molèsties.
Con motivo del cierre de verano, la validación de documentos se reanudará a partir del 28 de agosto de 2026. Disculpad las molestias
Due to the summer closure, document validation will resume starting August 28, 2026. We apologize for any inconvenience.

Fitxers

694739.pdf (638.09 KB)

Tipus de document

Article

Versió

Versió publicada

Data de publicació

2019-08-14

Llicència de publicació

cc-by (c) Comella-del-Barrio, Patricia et al., 2019
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/165112

A model based on the combination of ifn-γ, ip-10, ferritin and 25-hydroxyvitamin D for discriminating latent from active tuberculosis in children

Títol de la revista

Autors

Director/Tutor

ISSN de la revista

Títol del volum

Recurs relacionat

https://doi.org/10.3389/fmicb.2019.01855

Resum

In recent years, pediatric research on tuberculosis (TB) has focused on addressing new biomarkers with the potential to be used as immunological non-sputum-based methods for the diagnosis of TB in children. The aim of this study was to characterize a set of cytokines and a series of individual factors (ferritin, 25-hydroxyvitamin D [25(OH)D], parasite infections, and nutritional status) to assess different patterns for discriminating between active TB and latent TB infection (LTBI) in children. The levels of 13 cytokines in QuantiFERON-TB Gold In-Tube (QFT-GIT) supernatants were analyzed in 166 children: 74 with active TB, 37 with LTBI, and 55 uninfected controls. All cytokines were quantified using Luminex or ELISA. Ferritin and 25(OH)D were also evaluated using CLIA, and Toxocara canis Ig-G antibodies were detected with a commercial ELISA kit. The combination of IP-10, IFN-γ, ferritin, and 25(OH)D achieved the best diagnostic performance to discriminate between active TB and LTBI cases in children in relation to the area under receiver operating characteristic (ROC) curve 0.955 (confidence interval 95%: 0.91-1.00), achieving optimal sensitivity and specificity for the development of a new test (93.2 and 90.0%, respectively). Children with TB showed higher ferritin levels and an inverse correlation between 25(OH)D and IFN-γ levels. The model proposed includes a combination of biomarkers for discriminating between active TB and LTBI in children to improve the accuracy of TB diagnosis in children. This combination of biomarkers might have potential for identifying the onset of primary TB in children.

Matèries

Tuberculosi, Pediatria, Marcadors bioquímics, Resposta immunitària

Matèries (anglès)

Tuberculosis, Pediatrics, Biochemical markers, Immune response

Citació

Col·leccions

Articles publicats en revistes (Fonaments Clínics)

Citació

COMELLA-DEL-BARRIO, Patricia, et al. A model based on the combination of ifn-γ, ip-10, ferritin and 25-hydroxyvitamin D for discriminating latent from active tuberculosis in children. Frontiers in Microbiology. 2019. Vol. 10, num. 1855. ISSN 1664-302X. [consulted: 4 of August of 2026]. Available at: https://hdl.handle.net/2445/165112

Exportar metadades

JSON - METS

Compartir registre