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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/207947
Nasopharyngeal microbiota profiling of pregnant women with SARS-CoV-2 infection.
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We aimed to analyze the nasopharyngeal microbiota profles in pregnant women with and without SARS-CoV-2 infection, considered a vulnerable population during COVID-19 pandemic. Pregnant women were enrolled from a multicenter prospective population-based cohort during the frst SARS-CoV-2 wave in Spain (March-June 2020 in Barcelona, Spain) in which the status of SARSCoV-2 infection was determined by nasopharyngeal RT–PCR and antibodies in peripheral blood. Women were randomly selected for this cross-sectional study on microbiota. DNA was extracted from nasopharyngeal swab samples, and the V3-V4 region of the 16S rRNA of bacteria was amplifed using region-specifc primers. The diferential abundance of taxa was tested, and alpha/beta diversity was evaluated. Among 76 women, 38 were classifed as positive and 38 as negative for SARS-CoV-2 infection. All positive women were diagnosed by SARS-CoV-2 IgG and IgM/IgA antibodies, and 14 (37%) also had a positive RT–PCR. The overall composition of the nasopharyngeal microbiota difer in pregnant women with SARS-CoV-2 infection (positive SARS-CoV-2 antibodies), compared to those without the infection (negative SARS-CoV-2 antibodies) (p = 0.001), with a higher relative abundance of the Tenericutes and Bacteroidetes phyla and a higher abundance of the Prevotellaceae family. Infected women presented a diferent pattern of microbiota profling due to beta diversity and higher richness (observed ASV< 0.001) and evenness (Shannon index < 0.001) at alpha diversity. These changes were also present in women after acute infection, as revealed by negative RT–PCR but positive SARS-CoV-2 antibodies, suggesting a potential association between SARS-CoV-2 infection and long-lasting shift in the nasopharyngeal microbiota. No signifcant diferences were reported inmild vs. severe cases. This is the frst study on nasopharyngeal microbiota during pregnancy. Pregnant women with SARS-CoV-2 infection had a diferent nasopharyngeal microbiota profle compared to negative cases.
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CROVETTO, Francesca, SELMA-ROYO, Marta, CRISPI BRILLAS, Fàtima, CARBONETTO, Belén, PASCAL CAPDEVILA, Rosalia, LARROYA, Marta, CASAS, Irene, TORTAJADA, Marta, ESCUDERO, Nuria, MUÑOZ ALMAGRO, Carmen, GÓMEZ ROIG, Ma. dolores, GONZÁLEZ TORRES, Pedro, COLLADO, Maria carmen, GRATACOS, Eduard. Nasopharyngeal microbiota profiling of pregnant women with SARS-CoV-2 infection.. _Scientific Reports_. 2022. [consulta: 26 de febrer de 2026]. ISSN: 2045-2322. [Disponible a: https://hdl.handle.net/2445/207947]