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2015-12-12

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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/63186

The L-type voltage-gated calcium channel modulates microglial pro-inflammatory activity

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http://dx.doi.org/10.1016/j.mcn.2014.12.004

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Under pathological conditions, microglia, the resident CNS immune cells, become reactive and release pro-inflammatory cytokines and neurotoxic factors. We investigated whether this phenotypic switch includes changes in the expression of the L-type voltage-gated calcium channel (VGCC) in a rat model of N-methyl-d-aspartate-induced hippocampal neurodegeneration. Double immunohistochemistry and confocal microscopy evidenced that activated microglia express the L-type VGCC. We then analyzed whether BV2 microglia express functional L-type VGCC, and investigated the latter's role in microglial cytokine release and phagocytic capacity. Activated BV2 microglia express the CaV1.2 and CaV1.3 subunits of the L-type VGCC determined by reverse transcription-polymerase chain reaction, Western blot and immunocytochemistry. Depolarization with KCl induced a Ca2+ entry facilitated by Bay k8644 and partially blocked with nifedipine, which also reduced TNF-α and NO release by 40%. However, no nifedipine effect on BV2 microglia viability or phagocytic capacity was observed. Our results suggest that in CNS inflammatory processes, the L-type VGCC plays a specific role in the control of microglial secretory activity.

Matèries

Micròglia, Canals de calci, Sistema nerviós central, Malalties neurodegeneratives

Matèries (anglès)

Microglia, Calcium channels, Central nervous system, Neurodegenerative Diseases

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Articles publicats en revistes (Ciències Fisiològiques)

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ESPINOSA PARRILLA, Juan francisco, MARTÍNEZ MORENO, Martín, GASULL CASANOVA, Xavier, MAHY GEHENNE, Josette nicole, RODRÍGUEZ ALLUÉ, Manuel josé. The L-type voltage-gated calcium channel modulates microglial pro-inflammatory activity. _Molecular and Cellular Neuroscience_. 2015. Vol. 64, núm. 104-115. [consulta: 19 de desembre de 2025]. ISSN: 1044-7431. [Disponible a: https://hdl.handle.net/2445/63186]

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